黄芪多糖保护糖尿病心肌的初步研究  

Protective effect of astragalus polysaccharides on diabetic myocardium in hamsters

作　　者：陈蔚[1] 俞茂华[1] 叶红英[1] 

机构地区：[1]复旦大学附属华山医院老年科,上海200040

出　　处：《复旦学报（医学版）》2007年第4期541-544,548,共5页Fudan University Journal of Medical Sciences

基　　金：上海市科学委员会科研计划项目(05ZR14024)

摘　　要：目的观察黄芪多糖(APS)对糖尿病(DM)仓鼠心肌chymase/ACE-AngⅡ的影响,探讨其对DM心肌病变的干预机制。方法检测APS治疗组和DM对照组仓鼠的血糖、胰岛素、C肽、心肌酶谱、糖化血清蛋白水平,血浆和心肌组织的AngⅡ含量;免疫组化法检测心肌Ⅰ、Ⅲ型胶原;荧光定量PCR法检测心肌chymase和ACE mRNA表达;放免法检测chymase和ACE活性。结果APS组血糖、糖化血清蛋白、心肌酶谱和心肌AngⅡ水平较DM组显著下降,胰岛素、C肽、血浆AngⅡ水平和DM组无差异;APS组心肌Ⅰ型胶原表达和Ⅰ/Ⅲ型胶原比值较DM组显著下降;APS组chymase mRNA表达和活性均显著低于DM组,ACE基因的表达和活性与DM组无显著性差异。结论APS可以抑制糖尿病心肌中chymanse依赖性AngⅡ的生成,起到对糖尿病心肌病变的保护作用。Purpose To investigate the therapeutic value and possible mechanism of astragalus polysaccharide （APS） for the treatment of diabetic cardiomyopathy. Methods In APS group and diabetes mellitus（DM） control group, levels of serum glucose, insulin, C-peptide, myocardial enzymes, glycosylated serum protein （GSP） and angiotensin Ⅱ （Ang Ⅱ ） in plasma and myocardial were tested; Immuno-histochemistry was used to measure expression levels of type Ⅰ and type Ⅲ collagen; Expression levels of chymase Mrna and angiotensin-converting enzyme （ACE） mRNA were detected by using fluo- rescent quantitative polymerize- chain- reaction （FG-PCR） ; Activities of chymase and ACE were tested by radioimmunoassay. Results As compared to DM control group, levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in APS group, while levels of insulin, C-peptide and plasma Ang Ⅱ were the same. Levels of expression of collagen I and the ratio of collagen Ⅰ/collagen Ⅲ in APS group were lower than those in DM group. APS group showed lower levels of chymase mRNA expression and activity than those in DM control group,while there was no diference in ACE mRNA expression and activity between the two groups. Conclusions APS is effective in treating diabetic cardiomyopathy,with inhibition of chymase relied Ang Ⅱ in diabetic cardiomyopathy.

关 键 词：黄芪多糖 糖尿病心肌病变 胶原 胃促胰酶 血管紧张素Ⅱ 

分 类 号：R965.2[医药卫生—药理学]