硫酸镁对大鼠百草枯中毒肺损伤的干预及对COX-2的表达影响  

Effect of magnesium sulfate on lung injury and expression of COX-2 in the rat model of paraquat intoxication

作　　者：李俊英[1] 何庆[2] 

机构地区：[1]四川省人民医院急诊科 [2]四川大学华西医院急诊医学科教授

出　　处：《世界急危重病医学杂志》2007年第5期2036-2037,共2页internationl journal of emergency and critical care medicine

摘　　要：[论文特点介绍]本研究探讨硫酸镁对百草枯中毒肺损伤的干预作用及可能的干预机制，以期探讨百草枯中毒肺损伤的病理过程及可能的发展方向。COX-2通过在早期参与炎症反应和后期促进微血管增生参与百草枯中毒肺泡炎和肺纤维化的病理生理过程。Background and objective Paraquat intoxication can cause lung injury, with character of alveolar dropsy, pulmonary hemorrhage early. It can develop to pulmonary interstitial fibrosis later.There is no specific antidote for paraquat intoxication. COX-2 is known to be involved in pathology process of inflammation and angiogenesis. It has an important role in asthma, lung injury, lung cancer and lung fibrosis. Magnesium sulfate as a calcium antagonist can dilate spasm of microvessel and arteriole caused by lung hypoxia, to improve hypoxia and respiratory function. In addition, magnesium sulfate could arrest the tendency and activation of eosinophile granulocyte, increase stabilization of cell membrane, inhibit deregulation of mast cell, and thus educe anti-inflammation effect. However, there is not any study about the effect of magnesium sulfate on the lung injury induced by paraquat intoxication. We aimed to investigate whether magnesium sulfate could affect paraquat intoxication induced pulmonary injury of rats and its probable mechanism, to explore the pathology and development direction. Methods Sixty male SD rats were assigned to three different groups： paraquat intoxication model group （PQ group）; magnesium sulfate therapy group （MS group）; normal controlled group （NS group）. PQ group was given paraquat at a dose of 80 mg/kg by intragastric administration for once, and then douched normal saline everyday. MS group was given paraquat at dose of 80 mg/kg by intragastric administration for once, and then douched magnesium sulfate （30 mg/kg） everyday. NS group was given equivalent volume of normal saline. Measurements were determined at 3rd, 7th, 14th and 21st days post exposure. Measured lung coefficient in order to know about the level of pulmonary edema, and observed the pathological changes of lungs in the microscope. The expression of COX-2, CD-34 and collagen fiber by immnohistochemistry and the level of Ca^2＋-ATP in lung homogenate were measured. Results 1.The lung structure of magnesi

关 键 词：百草枯中毒 COX-2 肺损伤 硫酸镁 大鼠 病理生理过程 微血管增生 微血管生成 

分 类 号：R595.4[医药卫生—内科学] R735.2[医药卫生—临床医学]