银杏酮酯对大鼠坐骨神经损伤后神经生长因子蛋白表达的影响  

Effects of extract of Ginkgo Biloba leaves(EGb50) on protein expression of nerve growth factor after sciatic nerve injury in rats

作　　者：林浩东[1] 王欢[1] 陈德松[1] 李继峰[1] 顾玉东[1] 

机构地区：[1]复旦大学附属华山医院手外科,上海200040

出　　处：《复旦学报（医学版）》2007年第5期737-740,共4页Fudan University Journal of Medical Sciences

基　　金：国家973重点基础研究发展计划项目(2003CB515305);上海市科学技术委员会科研计划项目(04dz19855)

摘　　要：目的研究银杏酮酯对大鼠坐骨神经损伤后神经生长因子(nerve growth factor,NGF)蛋白表达的影响。方法SD大鼠78只,随机分成正常组、损伤对照组与实验组,后两组切断右侧坐骨神经并缝合。实验组给予银杏酮酯每日200mg/kg溶于1mL生理盐水中灌胃,损伤对照组给予生理盐水1mL灌胃,正常组不做处理。分别于术后1、3、7、14、21及28d取吻合口远段的神经、相应节段的脊神经节及脊髓,应用免疫组织化学和图像分析的方法研究所取组织中NGF的表达并进行定量分析。结果实验组坐骨神经、脊神经节及脊髓中NGF蛋白免疫阳性区域面积和平均灰度值在术后7、14、21和28d明显高于对照组(P<0.05,P<0.01)。结论大鼠坐骨神经损伤后用银杏酮酯治疗,在早期可促使坐骨神经及相应节段脊神经节和脊髓组织中的NGF蛋白表达增加。Purpose To investigate the effect of extract of Ginkgo Biloba leaves （EGB50） protein expression of nerve growth factor （NGF） after sciatic nerve injury in rats. Methods Sciatic nerve injury was established by transaction of right sciatic nerve in 72 male Spragne Dawtey rats,which were randomly divided into two groups. The rats were given EGb50, in the experimental group and normal saline in the control group by gavage. At the same time,other 6 rats were employed as the normal control without any treatments. The distal part of injuried nerve,dorsal root ganglia and spinal cord were harvested 1,3,7,14,21 and 28 days postoperatively. Immunohistochemistry and image analysis were used to evaluate the expression of NGF. Results The expression of NGF in sciatic nerve,dorsal root ganglia and spinal cord was higher in the experimental group than that in the control group on the 7th, 14th,21st and 28th day（P〈0. 05,P〈0.01）. Conclusions EGb50, increases the expression of NGF after sciatic nerve injury in rats.

关 键 词：神经生长因子 坐骨神经 银杏酮酯 神经损伤 

分 类 号：R681.7[医药卫生—骨科学]