辐照大豆诱发突变及其突变体的检测  

MUTATION INDUCED BY γ IRRADIATION, ELECTRON BEAM AND BENZAMIDE IN SOYBEAN

作　　者：于少华[1] 李梦[1] 李国全[1] 于海[1] 朴铁夫[1] 原亚萍[1] 陈光[1] 许耀奎[1] 

机构地区：[1]吉林农业大学

出　　处：《核农学报》1997年第3期129-134,共6页Journal of Nuclear Agricultural Sciences

基　　金：吉林省科委基础研究

摘　　要：不同剂量的γ射线、电子束单因子及其与不同浓度的苯甲酰胺复因子处理大豆种子，均能诱发其生育期、孕性和株高等农艺性状的突变，同时还能诱发蛋白质、氨基酸等品质突变，而且复因子处理较单因子处理的突变频率高。此外，筛选出的不同类型的突变体，其过氧化物酶活性及过氧化物酶同工酶酶谱均有明显差异。因此，可应用过氧化物酶等同工酶酶谱分析法对突变体进行检测。Mutations in growth period, fertility, plant height and character of protein, amino acid have been induced by using single and combined treatment of γ ray, electron with benzamide to soybean seeds and variant mutants have been obtained. The result showed that the combined treatment increased the mutation frequency. Activity and spectrums of POD iszymes among the mutants are obviously different.

关 键 词：大豆 诱发突变 突变体 

分 类 号：S565.103.5[农业科学—作物学]