毛细管区带电泳法测定甜瓜蒂药材中葫芦素B的含量  

Determination of cucurbitacin B in Pedicellus Melo by capillary zone electrophoresis

作　　者：刘金丹[1] 孙国祥[1] 池剑玲[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中成药》2008年第4期570-573,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金重大研究计划资助项目(90612002)

摘　　要：目的：建立毛细管区带电泳法（CZE）测定甜瓜蒂药材中葫芦素B含量的分析方法。方法：采用石英毛细管（75cm×75 μmI．D．），以50mmol／L硼砂溶液（含5％乙腈）为背景电解质，运行电压12．0kV，紫外检测波长228nm，以盐酸氯丙那林为内标测定不同产地的甜瓜蒂药材中葫芦素B含量。结果：测定葫芦素B的线性范围为0．15—1．2mg／mL，以相对峰面积对浓度回归的方程为Y=0．0034x ＋ 0．058，相关系数r=0．9992（n=6）。平均回收率100．2％（RSD=2．2％）。结论：该法简便快速、重现性好、低消耗和低污染，可用于测定甜瓜蒂药材中葫芦素B的含量。AIM： To develop a new method to determine cucurbitacin B in Pedicellus Melo by capillary zone electrophoresis （CZE）. METHODS ： All the samples were analysed on a fused silica capillary （75 cm × 75 μm I. D. ） by using 50 mmoL/L sodium borate solution （containing 5% acetonitrile） as the background electrolyte with the running voltage at 12.0 kV and detection wavelength of 265 nm. And clorprenaline hydrochloride was applied as the internal standard. RESULTS ： The results showed a good linear correlation between relative peak area of cucurbitacin B （y） and its concentration （x） over the range of 0. 15 - 1.2 mg/mL. Regression equation was y = 0.003 4x ＋ 0. 058, r = 0. 999 7, and the average recovery for baicalin was 100.2% with the RSD at 2.11%. CONCLUSION： The method is rapid, simple, reproducible and produces low pollution. It serves as a novel way to determine cucurbitacin B.

关 键 词：毛细管区带电泳 甜瓜蒂 葫芦素B 

分 类 号：R284.1[医药卫生—中药学]