肝素结合改良医用聚氯乙烯材料的细胞毒性评价  

Cytotoxicity evaluation of heparin coated modified PVC material

作　　者：杨剑[1] 段维勋[1] 徐学增[1] 俞世强[1] 魏旭峰[1] 刘金成[1] 易定华[1] 

机构地区：[1]第四军医大学西京医院心脏外科,西安710032

出　　处：《北京生物医学工程》2008年第1期88-90,共3页Beijing Biomedical Engineering

基　　金："十五"国家科技攻关计划课题(2004BA720A12)资助

摘　　要：目的评价肝素结合改良聚氯乙烯材料的细胞毒性及临床应用安全性。方法对肝素结合改良聚氯乙烯、改良聚氯乙烯等材料进行体外细胞培养,通过四甲基偶氮唑盐比色(MTT)实验观察细胞的活性和相对增殖率。结果改良聚氯乙烯及肝素结合的改良聚氯乙烯在吸光度及细胞的相对增殖率方面,结果优于传统聚氯乙烯材料;MTT实验表明肝素结合方法未改变材料的相对增殖率。结论改良聚氯乙烯及肝素结合的改良聚氯乙烯较传统材料相比,具有更优的细胞相容性,毒性轻微,可安全应用于临床。Objective To evaluate the cytotoxicity and clinical use safety of the heparin coated modified PVC material. Methods By in vitro L-929 mouse fibroblast cells culture and MTT assay, the cytotoxicity and relative growth rate （RGR） of heparin coated modified PVC and modified PVC materials were evaluated. Results The OD value and RGR of the heparin coated modified PVC and modified PVC materials were superior to traditional PVC. MTT assay results showed the heparin coating technique didn＇ t affect the RGR of artificial material. Conclusion The heparin coated modified PVC material has good cyto-compatibility and can be safely applied to clinical use.

关 键 词：聚乙烯亚胺 聚氯乙烯 细胞毒性 四甲基偶氮唑盐比色法 

分 类 号：R318.08[医药卫生—生物医学工程]