AFLP分子标记技术在浙蒲2号种子纯度快速鉴定中的应用  

Application of AFLP markers in fast determination of seed purity in gourd,Lagenaria siceraria cv.Zhepu No.2

作　　者：王玲平[1] 戴丹丽[1] 吴晓花[1] 汪宝根[1] 李国景[1] 

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2008年第2期84-87,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅重大攻关项目(2005C12001)

摘　　要：以瓠瓜杂种一代浙蒲2号及其父、母本和品系J099为材料,建立了利用AFLP分子标记技术进行瓠瓜杂种纯度鉴定技术体系。从64对引物组合中筛选出了两对稳定扩增、多态性好、分辨率高的引物组合E-ACC/M-CTA和E-ACC/M-CTT,可有效区分父、母本和杂种一代,适宜用于浙蒲2号种子纯度快速鉴定。A set of AFLP molecular marker techniques for determining hybrid seed purity of gourd, Lagenaria siceraria ev. Zhepu No. 2 was established by analysis of their hybrid generation, parents and a breeding line J099. Two pairs of primer combinations of E-ACC/M-CTA and E-ACC/M-CTT from 64 pairs were selected, which produced steadily polymorphic bands. These two pairs of primer combinations can clearly distinguish their genetic relationship between hybrid generation and their parents, and can be used to determine the purity of the hybrid seed.

关 键 词：瓠瓜 杂交种 AFLP 纯度鉴定 

分 类 号：Q814[生物学—生物工程] S642[农业科学—蔬菜学]