肌动蛋白是多头绒泡菌细胞核骨架和染色体骨架的组成成分  

ACTIN IS A CONSTITUENT OF THE NUCLEAR MATRIX AND CHROMOSOME SCAFFOLD IN PHYSARUM POLYCEPHALUM

作　　者：曾宪录[1] 焦明大[1] 王晓光[2] 郝水[1] 

机构地区：[1]东北师范大学遗传与细胞研究所,长春130024 [2]长春师范学院生物系,长春130032

出　　处：《Acta Botanica Sinica》1997年第8期691-696,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金重点项目(No.39131007)

摘　　要：自多头绒泡菌(Physarum polycephalum Schw.)的原质团中分离细胞核和染色体,分别经DNaseⅠ消化和2 mol/L NaCl抽提后制备成细胞核骨架和染色体骨架。以抗肌动蛋白的抗体作一抗、FITC标记的羊抗兔IgG抗体作二抗进行的间接免疫荧光实验结果显示,细胞核骨架和染色体骨架都分别与抗体呈阳性反应。间接免疫斑点印迹实验结果进一步证实,细胞核骨架和染色体骨架的蛋白质成分中存在与肌动蛋白抗体呈阳性显色反应的抗原。以抗肌动蛋白的抗体作一抗、金颗粒标记的蛋白A作二抗的间接免疫电镜实验结果表明,在实验组间期细胞核的核仁、集缩染色质和核基质以及中期染色体上都有很多金颗粒分布。上述结果证明,肌动蛋白是多头绒泡菌细胞核和染色体及其骨架的组成成分。Nuclear matrices and chromosome scaffolds were obtained by digesting and extracting, respectively with DNase I and 2 mol/L NaCl, the nuclei and chromosomes isolated from the plas-modia of Physarum polycephalum Schw. The results of the indirect immunofluorescence of rabit anti-actin antibody as immunomarker indicated that the nuclear matrices and chromosome scaffolds both had positive reaction with the antibody. The results of the indirect immunodotting experiment further verified the presence of actin antibody in their constituent. Protein A-colloidal gold immunoelectron microscopy technique revealed that gold particles were distributed in the interphase nuclei and metaphase chromosomes. The above results showed that actin is a constituent of the nuclear matrix and chromosome scaffold of P. polycephalum.

关 键 词：多头绒泡菌 肌动蛋白 细胞核骨架 染色体骨架 

分 类 号：Q949.320.2[生物学—植物学]