铁皮石斛种子的超低温保存研究  

CRYOPRESERVATION OF SEEDS OF DENDROBIUM CANDIDUM

作　　者：张治国[1,2] 刘骅 夏志俊[1,2] 王君晖 

机构地区：[1]浙江省医学科学院药物研究所 [2]杭州大学生命科学院

出　　处：《安徽中医学院学报》1997年第5期40-42,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金

摘　　要：研究影响铁皮石斛种子超低温保存的一些主要因素：种子含水量、解冻方法、恢复生长方法、保存时间等。结果表明，含水量是超低温保存的主要因素，种子含水量降到８％～１８％，直接投入液氮中，经保存后，４０℃水浴上快速解冻，种子萌发率为９２％～９５％，与未经冷冻的种子的萌发率一致，并生长成正常植株。Dendrobium candidum is one of preciouse rare and threatened medicinal plants.Its germplast preservation is very important for resource preservation and mass cultivation.The effects of seed water content,thawing methods,subculture methods,storage time on germination rate of seeds stored in liquid nitrogen(LN) were studied.The results showed that seed water content is key factor for cryopreservation in LN.The seeds were dried to water content 8%～18% before being put into LN for a period of time,and then thawed in 40℃ water bath for 1 minute.The germination rate of seeds reached 92%～95%.Seeds stored in LN could normally formed plantlets in suitable culture medium.

关 键 词：铁皮石斛 种子 超低温保存 液氮 

分 类 号：R282.4[医药卫生—中药学]