新型骨髓干细胞富集材料的制备及其表征研究  

Preparation and Measurement of a New kind of Scaffolds for Enriching Bone Marrow Stem Cells

作　　者：刘曦明[1] 许建中[1] 陈庄洪[2] 李世普[3] 罗飞[1] 曾玲[1] 刘杰[1] 

机构地区：[1]第三军医大学西南医院全军矫形外科中心,重庆400038 [2]广州军区武汉总医院骨科 [3]武汉理工大学生物材料与工程研究中心

出　　处：《华南国防医学杂志》2008年第2期9-11,共3页Military Medical Journal of South China

基　　金：国家高技术研究发展计划(863计划)重大攻关课题(2006AA02A122)

摘　　要：目的制备一种新型骨髓干细胞富集材料,并观察其表征。方法用预先制备的人脱钙骨基质(DBM)与多聚左旋赖氨酸(PLL)复合制备富集材料。参照Zhang氏液体置换法测定其密度与孔隙率;三维视频显微镜、扫描电镜观察其孔径、表面及横断面超微结构;拉曼光谱分析鉴定材料成分,并进行组织学观察。结果经PLL修饰的DBM富集材料(PLL-DBM)密度为(0.27±0.02)g/ml,孔隙率为(73±11)%,孔径为(412.73±160.29)μm。孔隙内部有大量孔隙相互连通,PLL在材料内外表面形成均匀的乳白色涂层,并在天然孔隙内形成更小、孔径较均匀的网孔结构。结论制备的PLL-DBM具有自体骨三维空间结构和促进细胞粘附的PLL,是一种较理想的骨髓干细胞富集材料。Objective To prepare and measure a new kind of scaffold for enriching bone marrow stem cells by selective cell retention. Methods Decalcified bone matrix （DBM） was decorated with poly-L-Lysine （PLL） as the scaffold material （PLL-DBM）. Porosity and density were measured with Zhang＇s process, Pore size, surface and cross section were observed under three-dimension video microscope and scanning electron microscope. Material component were analysized by Raman spectrum and histological observation. Results The density, porosity and pore size of PLL-DBM were （0. 27± 0. 02）g/ml, （73 ± 11）% and （412.73 ± 160.29）μm respectively. DBM were covered with uniformly-distributed and ivory white PLL, The PLL-DBM had high porosity with much more interior cross-connection pore. Conclusion The new constructed PLL-DBM has as good three-dimension space as autogenous bone, and well decorated with PLL for facilitating cells adhesion. It is an effective material for enriching bone marrow stem cells.

关 键 词：组织工程 脱钙骨基质 多聚左旋赖氨酸 选择性细胞滞留 骨髓干细胞 

分 类 号：Q67[生物学—生物物理学]