PLL-DBM支架材料富集骨髓有核细胞的效果评价  

Effects of Decalcified Bone Matrix Decorated with Poly-l-lysine on Enriching Bone Marrow Nucleated Cells by Selective Cell Retention

作　　者：刘曦明[1] 许建中[1] 陈庄洪[2] 罗飞[1] 曾玲[1] 刘杰[1] 叶青[1] 

机构地区：[1]第三军医大学西南医院全军矫形外科中心,重庆400038 [2]广州军区武汉总医院骨科

出　　处：《华南国防医学杂志》2008年第2期12-14,共3页Military Medical Journal of South China

基　　金：国家高技术研究发展计划(863计划)重大攻关课题(2006AA02A122)

摘　　要：目的评价PLL-DBM支架材料富集骨髓有核细胞的效果。方法用人脱钙骨基质(DBM)与不同浓度的多聚左旋赖氨酸(PLL)复合制备PLL-DBM支架材料。实验分组:A组:单纯DBM,B～F组分别是由0.01%,0.05%,0.1%,0.5%和1%的PLL与DBM制备的PLL-DBM。应用选择性细胞滞留技术观察PLL-DBM对骨髓有核细胞(NCs)的浓度富集倍数与粘附率。结果①PLL-DBM支架材料对人骨髓NCs浓度富集倍数与粘附率随着PLL浓度的增大而逐渐增加。②D组对人骨髓NCs浓度富集倍数为(3.18±0.31)倍,粘附率达(53±12)%,与A、B、C组比较,差异均有统计学意义(P<0.05);与E、F组比较,差异无统计学意义(P>0.05)。结论PLL-DBM支架材料对骨髓有核细胞的富集效果与PLL浓度间存在一定的量效关系,随着PLL浓度的增大而逐渐增加;0.1%PLL制备的PLL-DBM(D组)在富集效果和制备成本上分别优于其它各组,是较理想的骨髓干细胞富集材料之一。Objective To evaluate the effect of decalcified hone matrix（DBM） decorated with poly-l-lysine （PLL） on enriching bone marrow nucleated cells by selective cell retention. Methods DBM were decorated with PLL as the scaffold materials. According to the different PLL concentration, scaffolds were divided into 6 groups： group A （control group） was DBM group, group B, C, D, E, F were DBM decorated with 0. 01%, 0. 05%, 0. 1%, 0. 5% and 1% PLL respectively. With the selective cell retention technology （SCR）, the fold increase in concentration and the binding efficiency for bone marrow nucleated cells （NCs） of PLL-DBM were calculated. Results As PLL concentration in preparing PLL-DBM was increased, the fold increase in concentration and the binding efficiency for human bone marrow NCs were enhanced gradually. The fold increase in concentration and the binding efficiency in group D were 3.18±0. 31 fold and （53 ± 12）% respectively. There was significant difference compared with group A, B and C （P〈0.05）, but had the similar effect as in group E and F （P〉0.05）. Conclusion There is a dose-effect relationship between the effect of PLL-DBM on enriching bone marrow NCs increases and the increase of PLL concentration. DBM decorated with 0.1% PLL （group D） is superior to other groups in the effect of concentration and the preparation cost, so it is a kind of effective enriching material for bone marrow stem cell.

关 键 词：脱钙骨基质 多聚左旋赖氨酸 选择性细胞滞留 骨髓有核细胞 

分 类 号：Q67[生物学—生物物理学]