光谱法研究吲哚美辛,舒林酸及其金属配合物与牛血清蛋白的相互作用  

Investigation for the Interaction of Indomethacin,Sulindac and Their Metal Complexes with Bovine Serum Albumin by Liqand Displacement Reaction

作　　者：杨昌英[1] 刘义[2] 朱军成[2] 刘松秀[1] 

机构地区：[1]三峡大学化学与生命科学学院,宜昌443002 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析化学》2008年第4期473-477,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20543004,30570015,20621502);湖北省创新群体(No.2005ABC02);教育部创新团队发展计划(No.[2006]8IRT0543)资助项目

摘　　要：以曙红Y(Eosin Y)为探针,采用竞争结合法考查了非甾体抗炎药吲哚美辛和舒林酸及其相关的金属配合物与BSA的结合。在中性环境中,曙红Y的吸收光谱随着BSA的加入发生明显变化,结果表明,曙红Y与BSA之间的结合以静电作用为主,两者之间可能发生电子转移。曙红Y与BSA结合后,抗炎药吲哚美辛,舒林酸的加入可以破坏这种结合,曙红Y部分游离出来,其吸收值恢复,说明药物可以同样的方式结合到BSA上,抢占曙红Y在BSA上的结合位点。将药物转化为金属配合物后,与血清蛋白的亲和程度更强,铜(Ⅱ)的配合物最为突出,推测将非甾体抗炎药转化为铜配合物后,有更好的药理活性。A displacement approach was developed for the competitive binding studies. The binding reaction of non-steroidal anti-inflammatory drugs （ NSAIDs）, indomethacin, sulindac and their metal complexes with BSA were studied by using Eosin Y （ 2,4,5,7-tetrabromofluorescein ） as the potentiontric probe. It was showed that the addition of drug could displace Eosin Y which bound to BSA with electrostatic forces, and the absorbance of eosin Y changed toward free from eosin Y BSA complex. The competitive binding approach was used to access and compare quantitatively the displacing ability of the various drugs. The results indicated that Cu （ Ⅱ ） complex of indomethacin or sulidac showed more potential binding activity.

关 键 词：曙红Y 吲哚美辛 舒林酸 金属配合物 牛血清蛋白 竞争结合 

分 类 号：R96[医药卫生—药理学]