基于金纳米粒子/1,6-己基二硫醇组装的脱氧核糖核酸电化学传感器检测凝血因子Ⅻ片段  

Deoxyribonucleic Acid Electrochemical Biosensor Based on Self-assembled Au Nano-particles/1,6-Hexanethiols for the Detection of Blood Coagulation Ⅻ Segment

作　　者：张玉梅[1] 张雯艳[2] 朱果逸[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析国家重点实验室,长春130022 [2]吉林省妇幼保健院,长春130061

出　　处：《分析化学》2008年第4期478-482,共5页Chinese Journal of Analytical Chemistry

摘　　要：将含有17个碱基对的凝血因子Ⅻ单链脱氧核糖核酸(DNA)片段固定在十六烷基三甲基溴化胺(CTMAB)保护的金纳米粒子/1,6-己基二硫醇/金电极上,以道诺霉素(DNR)为电活性指示剂,用于检测其互补序列。电化学阻抗谱(EIS)监测了整个组装过程,电极界面性质随组装层的改变而变化。循环伏安(CV)研究发现,DNR与单、双链DNA以不同的方式结合。通过微分脉冲伏安法(DPV)检测DNR的还原峰电流,获得DNA杂交的最佳条件为:杂交时间1h,道诺霉素的浓度为1.0×10-4mol/L,巯基修饰的单链DNA(SH-ssDNA)组装时间为24h。在最佳杂交条件下,当互补DNA(cDNA)的浓度从1.0×10-13mol/L增加到1.0×10-8mol/L,DNR的还原峰电流与cDNA浓度的对数值呈线性关系,其线性回归方程为:y=0.288+0.022lgx,线性相关系数为0.9987,cDNA的检出限达8.1×10-14mol/L。The segment of blood coagulation XⅡ with 17 base pairs was immobilized on the surface of CTMAB- capped Au nanoparticles modified electrode for the detection of its complementary sequence with daunomycin （DNR） as electroactive indicator. The interracial properties of the electrode changed with the self-assembled monolayers （SAMs）. Daunomycin was bound to ssDNA and dsDNA with different interactions. The optimal conditions for DNA hybridization which was investigated by differential pulse voltammetry （DPV） was 1 h for hybridization time, 1.0 × 10 ^-4 moL/L DNR, and 24 h for the self-assembly of SH-ssDNA, respectively. The reduction peak current of DNR was linear with logarithm of the concentration of complementary DNA （cDNA） when it was changed from 1.0 × 10^-13 to 1.0 × 10^ -8 mol/L. The regression equation was y =0. 288 ＋0. 022 lgx and the regression coefficient （r） was 0. 9987. The detection limit of eDNA was 8.1 × 10^-14 mol/L.

关 键 词：凝血因子Ⅻ 金纳米粒子 道诺霉素 电化学阻抗 微分脉冲伏安法 化学修饰电极 

分 类 号：R446.1[医药卫生—诊断学]