葛根素对动脉粥样硬化兔髂动脉C反应蛋白表达的影响  

Effect of Puerarin on Expression of C-reactive Protein in Iliac Artery of Atherosclerotic Rabbit

作　　者：胡文志[1] 黄翘胜[2] 孙烈[1] 杨季明[1] 洪梅[1] 蒋振忠[1] 杨富[1] 张博晴[1] 周海波[1] 

机构地区：[1]南京医科大学第二附属医院心血管内科,江苏南京市210011 [2]郧阳医学院附属医院十堰市太和医院,湖北十堰市442000

出　　处：《中国康复理论与实践》2008年第4期321-322,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：南京医科大学科技发展基金重点项目资助(No.NYD2D18)

摘　　要：目的观察葛根素对动脉粥样硬化兔髂动脉分泌和表达C反应蛋白(CRP)的影响。方法20只家兔分为正常对照组(6只,正常饮食)、基础对照组(8只,球囊损伤和高脂饮食)和葛根素干预组(8只,球囊损伤、高脂饮食和葛根素干预),球囊损伤后4周处死;取一侧病变髂动脉做病理切片,应用免疫组化法测定CRP的表达;取另一侧病变髂动脉抽提总RNA,应用半定量逆转录多聚酶链式反应(RT-PCR)测定CRP mRNA的表达。结果葛根素干预组髂动脉粥样斑块CRP mRNA及其蛋白质表达均明显低于基础对照组(P<0.01)。结论葛根素可以通过调节兔动脉粥样斑块分泌CRP途径发挥稳定动脉粥样硬化斑块的作用。Objective To investigate the effect of Puerarin on expression of C-reactive protein （CRP） in iliae artery of atheroselerotic rabbits. Methods Twenty male New Zealand white rabbits were divided into the normal control group （n= 6, fed on a normal diet）, basic control group （n=8, fed on a cholesterol diet） and Puerarin group （n=8, fed on a cholesterol diet）. The balloon injury of lilac arteries was performed in animals of the basic control group and Puerarin group, and the animals of the Puerarin group were injected with Puerarin （25 mg/kg/d per rabbit） after injury. Four weeks after balloon injury, untreated and balloon injury iliac arteries were harvested for immunocytochemical staining. The mRNA and protein expression of CRP were tested by RT-PCR and immunucytochemistry. Results The mRNA and expression of CRP in atheroseclerotic plaque of iliae artery in the Puerarin group was significantly lower than that in the basic control group （ P〈0.01）. Conclusion Puerarin can make atheroseclerotic plaque stable by regulating of the expression of CRP in iliac artery of atherosclerotic rabbit.

关 键 词：葛根素 动脉粥样硬化 C反应蛋白 

分 类 号：R543.5[医药卫生—心血管疾病]