葡萄糖浓度对转人肿瘤坏死因子-α鱼腥藻IB02培养过程的影响  

Effect of Glucose Concentration on Cultivation Process of Transgenic Anabaena sp.PCC IB02 Harboring with Human Tumor Necrosis Factor-α

作　　者：朱学义[1] 张明华[1] 章华西[1] 张芃芃[2] 宋东辉[1] 施定基[1] 

机构地区：[1]天津科技大学海洋科学与工程学院,天津300457 [2]中国科学院植物研究所,北京100093

出　　处：《生物技术通讯》2008年第2期255-258,共4页Letters in Biotechnology

基　　金：天津市科委科技攻关重点项目(3015080)

摘　　要：目的:通过在培养基中添加葡萄糖的方法,提高转基因鱼腥藻的产量和人肿瘤坏死因子(hTNF)-α的表达率。方法:在葡萄糖浓度为0～300mmol/L的范围内,进行了转hTNF-α鱼腥藻IB02的摇瓶混合营养培养,用比浊法和酶联免疫法测定转基因鱼腥藻的生长和hTNF-α的表达。结果:添加葡萄糖的藻液最高生长密度是未添加葡萄糖的3.5倍,且hTNF-α的表达率也提高至4倍。结论:在各种葡萄糖浓度下,葡萄糖的利用都不明显。Objective： To improve the biomass of transgenic Anabaena sp. PCC IB02 harboring human tumor necrosis factor-a（hTNF-a） gene and the expression efficience of hTNF-a by the method of adding the glucose to the BG-11（-N） medium. Methods： Mixotrophic cultivation was carried out in flasks with glucose concentrations in the range from 0 to 300 mmol/L in BG-11（-N） medium. The turbidimetric method and enzyme-linked immunosorbent assay（ELISA） method were used to measure the biomass and the expression efficience of hTNF-a. Results： The data showed that the cell density increased to 3.5 times of that in control sample, and the expression rate of hTNF-a increased to 2%, from 0.5% in no glucose. Conclusion： At different glucose concentrations, the glucose consumption was not obviously.

关 键 词：人肿瘤坏死因子-Α 转基因鱼腥藻 混合培养 葡萄糖 

分 类 号：Q943.2[生物学—植物学]