黄花蒿组织培养研究  

Research on Tissue Culture of Artemisia annua

作　　者：贾秀山[1] 廖宇静[1] 于飞飞[1] 杨燕池[1] 尹秋平 

机构地区：[1]西南大学农学与生物科技学院,农业部生物技术与作物品质改良重点开放实验室,重庆市作物遗传育种重点学科,重庆400716

出　　处：《生物技术通讯》2008年第2期259-262,共4页Letters in Biotechnology

摘　　要：目的:筛选黄花蒿组织培养的配方,以寻求黄花蒿生长的最佳自然条件。方法:在MS基本培养基中添加不同激素,制备不同培养配方,筛选黄花蒿愈伤组织诱导与分化的最适培养基配方。结果与结论:诱导黄花蒿愈伤组织形成的最佳培养基配方为MS+6-苄氨基嘌呤(6-BA)(2.0mg/L)+激动素(KT)(2.0mg/L);诱导黄花蒿愈伤组织分化的最佳培养基配方为MS+6-BA(1.0mg/L)+吲哚乙酸(IAA)(1.0mg/L)。叶片诱导愈伤组织出愈时间最慢,出愈率较低,但其愈伤组织为胚性愈伤组织,后期愈伤组织的分化率、出苗率都很高,且不易产生褐变;带腋芽的茎段诱导愈伤组织形成最快,但分化率不及叶片愈伤组织,易褐变,适于快速转入生根培养基中直接用于快繁;花序基本不形成肉眼可见愈伤组织,直接形成幼苗。Objective： To screen the formula of Artemisia annua tissue culture for the seeking of the best nature condition for A.annua growing. Methods： Adopt the MS basic culture medium with different hormone to screen the optimized culture medium formula for the induction and differentiation of callus. Results ＆ Conclusion： MS＋6-BA（2.0 mg/L）＋KT （2.0 mg/L） and MS＋6-BA（1.0 mg/L）＋IAA（1.0 mg/L） were the optimized culture medium formula for the induction and differentiation of callus, respectively. With the leaf blade inducing to form callus, it recovered slowest and the induction rate was slowly. But its callus was embryogenic callus, and later period it showed high rate of differentiation and seedlings emergence, and also was not easy to have brown. While the stem sections with axillaries bud form callus were exactly on the contrary, and they were suitable to take into the culture medium for taking root fast, directly to use for rapid propagation. The rachis didn＇t form the callus that naked eye obviously and directly product seedling.

关 键 词：黄花蒿 组织培养 愈伤组织 分化 外植体 培养基 药用植物 

分 类 号：Q943.1[生物学—植物学]