天冬总皂苷对麻醉犬脑血流量及脑血管阻力的影响  

Influence of total saponins from Asparagus cochinchinensis on cerebral blood flow and vascular resistance in anesthetized dogs

作　　者：刘建国[1] 陈海生[1] 徐从立[1] 沈阳[1] 

机构地区：[1]第二军医大学药学院天然药物化学教研室,上海200433

出　　处：《第二军医大学学报》2008年第4期431-434,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(20472113)~~

摘　　要：目的:研究天冬总皂苷灌胃给药对麻醉犬脑血流量(CBF)及脑血管阻力(CVR)的影响。方法:杂种犬30只,体质量(11±1.5)kg,雌雄兼用,随即分为5组(每组6只):阴性对照组(生理盐水5 ml/kg,灌胃给药)、阳性对照组(尼莫地平注射液300/lg/kg,iv)、天冬总皂苷按10、30、60 mg/kg分低中高3个剂量组,灌胃给药。犬用戊巴比妥钠30 mg/kg静脉注射麻醉,手术暴露右侧颈总动脉,用MFV-3200型电磁流量计测定CBF和CVR;MPA3000生物电放大器记录血压(SBP、DBP)和心率(HR)等指标。结果:与阴性对照组比较,天冬总皂苷30、60 mg/kg组于给药后5 min即能明显增加CBF并一直持续到120 min(P<0.01),CBF随剂量增加而增加,显示出有较好的量效关系;天冬总皂苷30、60 mg/kg组用药后CVR虽较用药前降低,但大部分时间点未达到统计学显著意义;天冬总皂苷10 mg/kg组CBF、CVR无明显变化。结论:天冬总皂苷灌胃给药有显著增加麻醉犬CBF的作用。Objective：To study the effect of total saponins on cerebral blood flow and vascular resistance in anesthetized dogs. Methods： Thirty hybrid dogs in either sex,with a body weight of （11±1.5） kg,were evenly randomized into 5 groups： negative control group（saline 5 ml/kg,ig）, positive control （nimodipine 300 μg/kg,iv）, and 3 groups treated with total saponins （low-dose group [ 10 mg/kg, ig], middle-dose group[30 mg/kg, ig], and high-dose group[60 mg/kg, ig]）. The dogs were anes thetized with intravenous pentobarbital sodium （30 mg/kg）. The right common carotid artery was exposed to measure the cere bral blood flow,cerebral vascular resistance,blood pressure and heart rate using the MFV-3200 electromagnetic flow meter and MPA-3000 bioelectricity signal-amplifier. Results： Compared with negative control, cerebral blood flow was significantly in creased in animals treated with asparagus root saponins（30 and 60 mg/kg, ig）during 5 and 120 min after drug administration （P〈0.01）. No significant effect on cerebral blood flow and vascular resistance was found in animals treated with asparagus root saponins（10 mg/kg）. Conclusion： Asparagus root saponins can increase cerebral blood flow in anesthetized dogs.

关 键 词：天冬总皂苷 麻醉 脑血流 脑血管阻力 血压 心率 

分 类 号：R285.5[医药卫生—中药学]