卵泡刺激素调节大鼠卵巢颗粒细胞中Smad2/Smad3蛋白的表达及磷酸化  

Effects of follicle stimulating hormone on the expression and phosphorylation of Smad2/Smad3 protein in rat ovarian granulosa cells

作　　者：卫晓红[1] 祁丽花[1] 徐健[1] 迟晓春[1] 董静霞[1] 

机构地区：[1]北京大学医学部人体解剖学与组织胚胎学系,北京100083

出　　处：《解剖学杂志》2008年第2期141-144,共4页Chinese Journal of Anatomy

基　　金：北京大学医学部985基金(612206062);教育部留学回国基金(413152015)

摘　　要：目的:研究卵泡刺激素(FSH)对大鼠卵巢颗粒细胞中Smad2/Smad3蛋白的调节。方法:21dSD雌鼠,腹腔注射孕马血清20IU,对颗粒细胞进行原代培养,用转化生长因子β受体Ⅱ(TβRⅡ)抗体中和卵巢颗粒细胞表面TβRⅡ,以阻断TGFβ/Smads信号通路,再以不同浓度的FSH对细胞进行不同时间的处理,免疫印迹法检测TGFβ信号通路中TβRⅡ、Smad2/Smad3及P-Smad2/P-Smad3蛋白的表达。结果:TβRⅡ、Smad2/Smad3、P-Smad2/P-Smad3蛋白的表达量与FSH的作用呈时间依赖性显著增强;TGFβ/Smads信号通路被阻断后再用FSH处理,Smad2/Smad3、P-Smad2/P-Smad3蛋白的表达量没有明显变化;细胞表面TβRⅡ恢复后,Smad2/Smad3、P-Smad2/P-Smad3蛋白的表达量有少量增加。结论:FSH刺激大鼠卵巢颗粒细胞中Smad2/Smad3的表达和磷酸化;FSH对Smad2/Smad3的激活作用在相当程度上依赖于TβRⅡ。Objective： To study the effects of follicle stimulating hormone （FSH） on the expression and phosphorylation of Smad2/Smad3 proteins and the interaction between FSH and TGF-β/Smads signaling pathway in rat ovarian granulosa cells （GCs）. Methods：The GCs from female SD rats of 21 d were cultured with TβRⅡ antibody and FSH at different concentration, and then cells were obtained at defined points to determine the expression of Smad2/Smad3 and P-Smad2/P-Smad3 by Western Blot assay. Results： FSH enhanced the expression of TβRⅡ, Smad2/Smad3 and P-Smad2/P-Smad3 proteins in manner of time dependent; Neutralization of TβRⅡ antibody to TβRⅡ on the surface of GCs inhibited the stimulation of FSH on Smad2/Smad 3 in GCs; The effects of FSH on Smad2/Smad3 proteins recovered when TβRⅡ was expressed again. Conclusion： In the culture condition, FSH stimulated the expression and phosphorylation of Smad2/Smad3; The activation of FSH on Smad2/Smad3 depended on TβRⅡ.

关 键 词：卵巢 颗粒细胞 卵泡刺激素 转化生长因子Β SMAD2 SMAD3 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]