冷冻连续切片重建数字人腰髓  

Reconstruction of digital human lumbar spinal cord based on serial sections

作　　者：吕广明[1] 吴辉群[1] 汤乐民[2] 韩笑[1] 季达峰[1] 顾晓松[1] 

机构地区：[1]南通大学医学院解剖学教研室,神经生物学研究所,江苏省神经再生重点实验室,南通226001 [2]南通大学医学院影像工程实验室,南通226001

出　　处：《解剖学杂志》2008年第2期232-234,共3页Chinese Journal of Anatomy

基　　金：江苏省高校自然科学基金(05KJB180105);江苏省研究生创新培养工程资助项目(CX07s_035z);江苏省神经再生重点实验室开放课题(JSK-04003);南通市社会发展基金(S40052)

摘　　要：目的:探讨数字人脊髓数字化问题,建立一种实用的脊髓三维重建方法。方法:对人腰髓节段的连续冷冻切片直接贴片,进行Luxol Fast Blue染色,采集切片图像并进行图像拼接,Photoshop 7·0软件对图像进行"图像灰度化"、"反相"、"对比度调整"、"图像缩小"等后处理,3D-DOCTOR 4·0软件进行表面重建和体重建。结果:重建出人腰髓节段及其内部的灰质结构具有立体感,并可在计算机上进行自由观察和测量。结论:利用人腰髓连续切片可以进行人体脊髓的三维重建。Objective： To explore the digitalization of human spinal cord in visible human and construct a useful method of three dimensional （3D） reconstruction of the spinal cord. Methods： The serial sections of human lumbar cord were directly mounted and then stained with luxol fast blue （LFB）, and the images were collected and stitched. The images were processed with ＂gray scale＂ , ＂invert＂ , ＂image size diminution＂ in Photoshop 7.0 software and then were done surface and volume reconstruction with 3D-DOCTOR 4.0 software. Results： The reconstructed human lumbar cord segment and its grey matter were three dimensional, and could be observed and measured freely. Conclusion： The 3D human spinal cord can be reconstructed with serial sections and used for further visible research on inner structure of the spinal cord.

关 键 词：虚拟人 连续切片 表面重建 luxol FAST BLUE 3D-DOCTOR 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]