RP-HPLC测定不同产地肉桂中桂皮醛和肉桂酸的含量  

Determination of Cinnamaldehyde and Cinnamic Acid in Cortex Cinnamomi from Different Habits by RP-HPLC

作　　者：马蓉蓉[1] 唐意红[2] 孙兆林[2] 季宇彬[1] 黄成钢[2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究发展中心,黑龙江哈尔滨150076 [2]中国科学院上海药物研究所,上海201203

出　　处：《中国现代中药》2008年第4期9-11,共3页Modern Chinese Medicine

基　　金：中国科学院重要方向项目(KGCX2-SW-213-03)

摘　　要：目的:建立不同产地肉桂中有效成分桂皮醛和肉桂酸的含量测定方法。方法:采用高效液相色谱法,测定了16个产地肉桂中桂皮醛和肉桂酸的含量。色谱柱:迪马公司DiamonsilTM(钻石)C18柱(250mm×4.6mm,5μm);检测波长:285nm;温度:20℃;流动相:乙腈-0.3(磷酸水溶液(35∶65);流速:1mL.min-1。结果:建立了同时测定肉桂中桂皮醛和肉桂酸的高效液相色谱法,产自越南的肉桂中桂皮醛和肉桂酸的含量明显高于其他产地。结论:本法简便、快速、准确,适用于肉桂中桂皮醛和肉桂酸的含量测定。Objective： To establish a cinnamomi from different habitats. Methods method for the determination of cinnamaldehyde and cinnamic acid in cortex ： A HPLC method was performed on a Diamonsil^TMC18 （250mm ×4. 6mm, 5μm） column. The mobile phase consisted of CH3CN-H2O（with 0. 3%H3PO4） （35：65） with the detection wavelength at 285nm. The column temperature was at 20℃ and the flow rate was 1.0mL·min^-1 Results： A HPLC method has been developed for the determination of cinnamaldehyde and cinnamic acid in cortex cinnamomi from different habitats. The sample from Vietnam, own the highest content of cinnamaldehyde and cinnamic acid. Conclusion： This method is simple, reliable, repeatable, and is suitable for the determination of cinnamaldehyde and cinnamic acid in cortex cinnamomi.

关 键 词：肉桂 桂皮醛 肉桂酸 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R284.1