L-肉碱预处理对离体家兔心肌缺血再灌注损伤的保护作用  

Protective effects of L-carnitine pretreatment on myocardial ischemia-reperfusion injury of isolated rabbit hearts

作　　者：郝传吉[1] 刘涛[1] 姚文[1] 王慧敏[1] 

机构地区：[1]青岛大学医学院附属医院,青岛266003

出　　处：《药学与临床研究》2008年第2期100-103,共4页Pharmaceutical and Clinical Research

摘　　要：目的:观察L-肉碱预处理对离体家兔心肌缺血再灌注损伤的保护作用。方法:采用离体兔心Lan-gendorff灌注实验模型,离体兔心24只,随机等分成3组(n=8)。正常对照组:连续灌注K-H液90min;缺血再灌注损伤组:灌注30min,关闭主动脉套管停止灌注,30min后恢复37℃K-H液灌注60min;L-肉碱组:步骤同缺血再灌注组,但在停灌前先用含10mmol·L-1L-肉碱的K-H液灌注30min。记录血流动力学指标:冠脉流量(CF)、左心室舒张压(LVDP)、左心室压力时间变化率(±dp/dt);检测冠脉流出液中丙二醛(MDA)、超氧化物歧化酶(SOD)、肌酸激酶(CK)、乳酸脱氢酶(LDH)浓度和心肌组织中MDA、SOD及L-肉碱的含量。结果:L-肉碱组能显著促进再灌注时心功能恢复:±dp/dtmax和LVDP显著升高;CF增大;冠脉流出液中MDA、LDH、CK以及心肌组织中MDA浓度降低;而冠脉流出液和心肌组织中SOD含量均升高(P<0.05);心肌中L-肉碱含量明显增高(P<0.01);超微结构观察结构损伤较轻(P<0.05)。结论:L-肉碱预处理具有抗兔离体心肌缺血再灌注损伤的作用。Objective： To investigate the protective effect of L-carnitine pretreatment against myocardial ischemia-reperfusion （I-R） injury in Langendorff isolated rabbit hearts. Methods：24 rabbit hearts were randomly divided into normal control group, ischemia-reperfusion injury group and L-carnitine group. The normal control group was perfused with Krebs-Henseleit buffer （K-H） solution for 90 min. The I-R injury group was firstly perfused with K-H solution for 30 min, followed by aorta occlusion for 30 min and then reperfused with K-H solution for 60 min. The L-carnitine group was similarly treated to that of the I-R group, except that 10 mmol·L^-1 of L-carnitine was added in to the K-H solution before ischemia. The hemodynamic indexes like coronary flow （CF）, left ventricular diastolic pressure （LVDP） and it＇s first derivative （ ± dp/dt） were recorded. The activity of maiondiaidehyde （MDA）, superoxide dismutase （SOD）, creatine kinase （CK） and lactic dehydrogenase（LDH） in coronary effluent and contents of MDA, SOD and L - carnitine in myocardial tissues were measured in vitro. Resuits ： L-carnitine caused a significant improvement of cardiac function （ LVP and ± dp/dtmax, increased significantly, and CK enhanced） and a decrease in the release of MDA, LDH and CK in coronary effluent as well as the level of MDA in myocardial tissues, the level of SOD were observed enhanced both in coronary effluent and myocardial tissues. Besides, the content of L-carnitine in muscle was increased （P 〈 0.01 ） and ultramicrostructure indicated a minor injury of heart muscle compared with the I-R injury group （P 〈 0.05 ）. Conclusion ： L-carnitine pretreatment possesses a protective effect against myocardial I-R injury of isolated rabbit hearts.

关 键 词：L-肉碱 缺血再灌注损伤 离体免心 

分 类 号：R965[医药卫生—药理学]