TRFIA与RIA检测AFP的对比分析  

Comparison between time - resolved fluorescence immunoassay and radioimmunoassay for detection alpha fetoprotein

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作  者:杨凤爱 林荣军 茹辽金 王征 邝敏健 Yang Fengai, Lin Rongjun, Ru Liaojin , Wang Zheng, Kuang Minjian (1. The Department of Laboratory Medicine, Yangjiang people' s hospital 529500 ; 2. Guangzhou Darui antibody Co, Ltd. 510663 )

机构地区:[1]阳江市人民医院,广东阳江529500 [2]广州达瑞抗体工程技术有限公司

出  处:《中国保健》2008年第11期388-390,共3页Chinese Health Care

摘  要:目的:应用TRFIA与RIA两种方法分别检测血清中AFP指标的含量,比较两种方法测定AFP的相关性。方法:分别用TRFIA与RIA两种方法检测正常对照组、肝硬化组、肝癌组三组AFP的含量,对相应结果进行统计分析。结果:肝癌组AFP含量TRFIA法:(352.18±308.32)ng/ml,RIA法:(318.68±286.12)ng/ml;肝硬化组AFP含量TRFIA法:(38.28±14.25)ng/ml,RIA法:(33.92±13.66)ng/ml;正常对照组AFP含量TRFIA法:(6.88±4.65)ng/ml,RIA法:(6,02±4.91)ng/ml,肝癌组分别与肝硬化组及正常对照组相比均有非常显著意义(P〈0.01),三组内两种方法测定结果相比均无显著意义(P〉0,05);两种方法相关性r=0.996(P〈0.001),相关性好;阳性符合率为96.6%,阴性符合率为95.1%,总符合率为95.6%。结论:两种方法均能满足临床对AFP检测的需求,在方法学上TRFIA要优于RIA。Objective:Serum alpha fetopmtein(AFP) levels were detected with beth timeresolved fluorescence immunoassay (TRFIA)and radioimmunoassay ( RIA), to evaluate the dependability of two methods for detecting the serum level of AFP. Methods:Serum AFP levels were detected with both TRFIA and RIA in samples from patients with the normal control group, hepatic cirrhosis group and hepatoma group ,the data were analyzed with statistical software. Results:The level of AFP was significantly higher in the hepatoma group TRFIA: (352. 18 ± 308. 32 )ng/ml, RIA: (318.68 ± 286. 12 )ng/ml than that in the hepatic cirrhosis group TRFIA: (38.28 ± 14. 25 )ng/ml, RIA: (33.92 ± 13.66 )ng/ml and in the normal control group TRFIA: ( 6. 88 ± 4. 65 ) ng/ml, RIA: ( 6. 02 ± 4. 91 ) ng/ml ( P 〈 0. 01 ). There was no significance difference between the two methods in three groups, respectively ( P 〉 0. 05 ) ; The dependability of two methods were well correlated ( r = 0. 996 ) ; The positive accordant ratio was 96.6%, and the negative accordant ratio was 95.1%, and the accordant ratio was 95.1%. Conclusion: Two methods were satisfied the demand of AFP in clinic ,TRFIA was better than RIA in the technology.

关 键 词:时间分辨荧光免疫分析 放射免疫分析 甲胎蛋白 

分 类 号:R446.61[医药卫生—诊断学;医药卫生—临床医学]

 

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