镍冶炼烟尘对NIH/3T3细胞吞噬活性的影响  

Effect of nickel-smelting fume on phagocytizing activity of NIH/3T3 cells

作　　者：金艳涛[1] 吴永会[1] 于翠平[1] 陈丽丽[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081

出　　处：《中国工业医学杂志》2008年第2期76-78,共3页Chinese Journal of Industrial Medicine

基　　金：国家自然科学基金(30571549);黑龙江省高等学校青年学术骨干支持计划项目(1152G020)

摘　　要：目的研究镍冶炼烟尘对NIH/3T3细胞的毒性。方法以我国某镍冶炼厂的镍冶炼炉前沉降尘和镍精矿车间沉降尘为受试物,观察NIH/3T3细胞对其的吞噬活性,并用噻唑蓝(MTT)比色的方法检测细胞活性。结果两受试物均可被NIH/3T3细胞吞噬,在200μg/ml时的吞噬率分别为100.0%、83.0%;100μg/ml时的吞噬率为77.0%、58.8%;50μg/ml时的吞噬率为59.8%、30.5%,差异均有统计学意义(P<0.05);且随作用时间增长,细胞生长受到抑制程度也随之增加。接触24h,两受试物细胞半数抑制浓度分别为19.86、30.15μg/ml。结论两受试物均可诱导NIH/3T3细胞吞噬活性,可以降低细胞的成活率,为镍冶炼烟尘的致癌危险性以及镍冶炼工肺癌病因学研究提供新的实验依据。Objective To study the toxicity of nickel-smelting fume on NIH/3T3 cells. Methods To observe the effect of two kinds of nickel fumes collected from smelting furnace and refining workshop of a nickel smeltery in China on the phagocytizing activity of NIH/3T3 cells; meanwhile, the cytoactivity was also detected with MTT colorimetric assay. Results It was shown that two kinds of nickel fumes all could be phagocytizied by NIH/3T3 cells. The phagocytizing rate was dose-dependent ： at the exposed level of 200μg/ml, the rates were 100. 0% and 83.0% ; and 77.0%, 58. 8% at 100μg/ml; and 59.8%, 30. 5% at 50μg/ml, respectively, there were significant differences. Additionally, with the extension of exposure time, the growth of NIH/ 3T3 cells was also inhibited. After 24 hours exposure, the 50% inhibiting concentrations of cell growth were 19. 86 and 30, 15μg/ml respectively for these two kinds of fumes. Conclusions Two kinds of the fumes all might induce the phagocytizing activity and depress the survival rate of NIH/3T3 ceils. These findings provide a new experimental evidence for the carcinogenic risk of nickel-smelting fume and the etiological agent of lung cancer in nickel-smeltlng workers.

关 键 词：镍 冶炼烟尘 吞噬活性 致癌性 

分 类 号：R994.6[医药卫生—毒理学] O614.813[医药卫生—药学]