实验性小肠移植早期排斥标志物研究  

Experimental study on marker of early rejection in small bowel transplantation

作　　者：王浩[1] 王鹏志[1] 王军[1] 朱理玮[1] 刘自宽[1] 

机构地区：[1]天津医科大学总医院外科,300052

出　　处：《中华器官移植杂志》1997年第4期226-228,共3页Chinese Journal of Organ Transplantation

摘　　要：为寻找小肠移植（SBT）早期排斥标志物，本实验采用逆转录聚合酶链式反应技术，对小鼠SBT术后12小时受体T细胞表面白细胞介素-2受体α链（IL－2Rα）编码基因进行定性检测，并对移植小肠行同期病理学检查。结果发现：同种异体移植小鼠体内IL-2RαcDNA阳性例数均明显高于非手术、同系移植及加环孢素A（CsA）治疗的同种异体移植小鼠；移植小肠病理学检查无异常变化。提示：T细胞表面IL－2Rα基因的活化是启动排斥反应的关键；CsA可抑制IL－2Rα基因转录；IL－2Rα编码基因可作为SBT术后早期排斥标志物。In order to investigate the marker of early rejection in small bowel transplantation (SBT), a chain coding gene of interleukin-2 recepto r(IL-2Rα) on the surface of T lymphocyte of recipients in mice was qualitatively assayed 12 h after operation by reverse transcription polymerase chain reaction (Rt-PCR). Pathological features of the grafts were simultaneously determined, The results showed that the level of IL-2Rα cDNA in allografts was significantly higher than in nonoperative, isograft and allograft being treated with CsA subgroups; No pathological changes in rejection were found in the grafts. It is concluded that activation of IL-2Rα coding gene is the key to induce rejection; CsA may inhibit the transcription of IL-2RQ coding gene; IL-2Rαcoding gene may be used as a marker of early rejection in small bowel transplantation.

关 键 词：小肠移植 白细胞介素2 排斥反应 

分 类 号：R656.705[医药卫生—外科学]