从噬菌体7肽库中筛选哇巴因特异性结合的短肽  

Screening of Conjugated Peptide of Ouabain from Phage Display Peptide Library

作　　者：徐忠伟[1] 徐瑞成[1] 陈小义[1] 刘英富[1] 

机构地区：[1]中国人民武装警察部队医学院细胞生物学教研室,天津300162

出　　处：《药物生物技术》2008年第2期104-108,共5页Pharmaceutical Biotechnology

基　　金：天津市自然科学基金资助项目(项目编号:06YFJMJC10400)

摘　　要：以哇巴因为靶分子,从噬菌体7肽库中筛选与哇巴因特异性结合的短肽。经过3轮淘筛并通过ELISA法鉴定,获得14个阳性克隆,通过对噬菌体ssDNA电泳鉴定和序列分析筛选得到3种短肽:肽A、B和C的筛选一致率分别为64.3%(9/14),28.6%(4/14)和7.14%(1/14)。Genbank中蛋白质同源性分析显示,肽A、B、C均不与钠泵蛋白同源。放射性配基受体结合法检测结果表明,合成的肽A能够与哇巴因结合。哇巴因特异性结合短肽的获得将为阻遏内源性哇巴因与钠泵的结合、防治高血压奠定基础。The ouabain was used as target to screen conjugated peptide of ouabain from Ph.D.-7 Phage Display Peptide Library. After 3 rounds of screening, the 14 positive phage clones were identified by the methods of phage ELISA and DNA electrophoresis analysis. The sequence of each selectett peptide was determined and analyzed through Internet Genbank. Three kinds of peptides were found out. Peptides A, B, and C were occupied in 64.3% （9/14）, 28. 6%（4/14） and 7.14%（1/14） respectively. The analysis of proteins A, B and C showed that there was no homology with sodium pump protein. The results of radioligand binding assay showed that there was binding activity between synthetical peptide A and ouabain. Obtaining the conjugated peptide of ouabain could lay a foundation for blocking the endogenous ouabain action on sodium pump and treating hypertension.

关 键 词：哇巴因 噬菌体展示肽库 筛选 

分 类 号：Q514.3[生物学—生物化学]