卡莫氟固脂纳米粒对人大肠癌细胞多药耐药的逆转的机制研究  

The Mechanism on Reversion of MDR in Human Carcinoma Line with HCFU-SLNs

作　　者：孟胜男[1] 王欣[1] 王怀良[2] 

机构地区：[1]中国医科大学药学院药剂学教研室,辽宁沈阳110001 [2]中国医科大学药学院临床药理教研室,辽宁沈阳110001

出　　处：《药物生物技术》2008年第2期120-123,共4页Pharmaceutical Biotechnology

基　　金：辽宁省教育厅科学研究计划资助项目(No.05L472)

摘　　要：研究卡莫氟固脂纳米粒对人大肠癌细胞多药耐药的逆转作用,并对其逆转机制进行了初步探讨。采用MTT法比较了卡莫氟不同药物形式的细胞毒作用;采用RT-PCR技术考察卡莫氟固脂纳米粒对敏感和耐药人大肠癌细胞中mdr 1和MRP的基因表达的影响。卡莫氟固脂纳米粒能下调耐药人大肠癌细胞中mdr 1的mRNA表达,对其中的MRP的mRNA的表达没有显著影响。卡莫氟固脂纳米粒能逆转人大肠癌细胞由mdr 1介导的多药耐药,而对MRP介导的多药耐药没有显著影响。To study reversal effect of MDR on human carcinoma line by HCFU-SLNs and its abecedarian mechanism. Cytotoxicity of free HCFU and HCFU-SLNs were determined by measuring the inhibition of cell growth using the MTT assay; the mRNA expression levels of mdrl and MRP in sensitive and drug-resistant human carcinom cells were quantified using reverse transcription polymerase chain reaction （RT - PCR）. HCFU-SLNs can down-regulate the mRNA expression level of mdrl of the drug-resistant human carcinom cells, and have no marked influence on its mRNA expression level of MRP. HCFU-SLNs were effective to reverse the multidrug resistance mediated MDR of human carcinoma cells, but have no effects on the multidrug resistance mediated MRP.

关 键 词：卡莫氟 固脂纳米粒 多药耐药 逆转 

分 类 号：R965[医药卫生—药理学]