二草清肝汤对大鼠免疫性肝损伤的保护作用及其机制  

A study on the protective effects and mechanisms of ercao qinggan tang to immunological liver injury in rats

作　　者：曹家麟[1] 吴春明[1] 朱小区[1] 欧阳钦[1] 苏林红[1] 

机构地区：[1]浙江省温州市中医院肝病科,温州325000

出　　处：《临床医学》2008年第3期109-111,共3页Clinical Medicine

摘　　要：目的观察中药二草清肝汤对大鼠免疫性肝损伤的保护作用及其机制的初步探讨。方法采用卡介苗(BCG)+脂多糖(LPS)注射制备大鼠免疫性肝损伤模型,共60只大鼠,设正常组、模型组、环磷酰胺(CTX)组、二草清肝汤小剂量组、二草清肝汤大剂量组,检测不同组血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转酶(AST)和肿瘤坏死因子-α(TNF-α)、白细胞介素-12(IL-12)的变化,流式细胞仪检测肝细胞凋亡,免疫组织化学方法检测肝细胞Bax、Bcl-2蛋白的表达。结果二草清肝汤大、小剂量组和CTX组与模型组比较,ALT及AST降低明显,ALT分别为(547±76)、(576±69)、(538±72)、(642±94)U/L,AST分别为(536±72)、(585±74)、(526±71)、(627±92)U/L,差异有统计学意义(P<0.01或P<0.05)。二草清肝汤治疗组和CTX组TNF-α、IL-12与模型组比较下降显著,差异有统计学意义(P<0.05)。二草清肝汤治疗组、CTX组与模型组比较,肝细胞凋亡率下降,差异有统计学意义(P<0.01或P<0.05);二草清肝汤治疗组、CTX组Bcl-2和Bax阳性表达及Bax/Bcl-2相对比率明显低于模型组,差异有统计学意义(P<0.01或P<0.05)。结论二草清肝汤对大鼠免疫性肝损伤具有保护作用,降低血清TNF-α、IL-12水平和抑制肝细胞凋亡可能是其作用机制之一。Objective To study the protection and mechanisms of ereao qinggan tang to immunological liver injury in rats. Methods The immunological liver injury rat model was produced by intraperitoneal injection with BCG plus LPS. Normal control group, immunological liver injury model group, Cyclophosphamide （CTX） group, ereao qinggan tang low dose group and ercao qinggan tang high dose group were set up. The levels of aminotransferase （ALT）, aspartate aminotransferase （AST）, tumor necrosis factor -α（ TNF -α） ,interleukin - 12 （ IL - 12） in blood plasma of the five groups were examined. The rates of apoptosis of hepatocytes were analyzed by flow cytometry. The expression of Bax and Bcl - 2 was detected qualitatively and quantitatively by immunohistochemistry assay using an image analysis system. Results The levels of ALT and AST in the ercao qinggan tang （ large dose group and small dose group） and CTX group were lower than those of the immunological liver injury model group. ALT was （ 547 ± 76）, （ 576 ± 69 ）, （ 538 ± 72 ）, （ 547 ± 76 ） U/L respectively and AST was （ 536 ± 72 ） , （ 585 ± 74 ）, （ 526 ± 71 ） , （627 ±92）U/L respectively. The differences between the ereao qinggan tang groups or CTX and the model group were significant （ P 〈0.01 orP 〈0.05）. The levels ofTNF-α and IL- 12 in the ercao qinggan tang groups and the CTX group were lower than those of the model group, and the differences were significant （ P 〈 0.05 ）. The rates of hepatocytes apoptosis of the ercao qinggan tang treatment group or the CTX group were lower than that of the model group, and the differences were significant （ P 〈0.01 or P 〈0.05 ）. The comparing ratios of Bax and Bcl- 2 proteins （Bax/Bcl- 2） expression in hepatocytes in ercao qinggan tang high dose group and CTX group were significantly lower than that of that the model （ P 〈 0.01 or P 〈0.05 ）. Condusison Ercao qinggan tang can decrease the levels of TNF -α and IL - 12 in plasma and inhibit

关 键 词：免疫性肝损伤 二草清肝汤 肿瘤坏死因子-Α 白细胞介素-12 细胞凋亡 

分 类 号：R285[医药卫生—中药学]