不同稀释液配方对奶牛冷冻精液的影响  

The Effects of Different Extenders on Dainy Cow Frozen Semen

作　　者：王立强[1] 胡建宏[1] 刘蝉铭[1] 李青旺[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《畜牧兽医杂志》2008年第3期17-19,22,共4页Journal of Animal Science and Veterinary Medicine

摘　　要：采用假阴道法采集成年种公牛精液,经精液品质检查后,合格精液用四种不同冷冻稀释液进行稀释、冷冻、以筛选出适宜的冷冻稀释液和最佳冷冻保护剂。结果表明:Ⅱ号稀释液优于其它3种稀释液(p<0.05);甘油为最佳冷冻保护剂。优于乙二醇和DMSO冷冻效果(p<0.05),其最佳浓度为7%,精子冷冻后活率平均可达0.45±0.575,畸形率15.44%,顶体完整率57.82%;奶牛细管冻精的解冻效果以75℃水浴,2 S到6 S的解冻时间最佳。The OX Semen were collected by Using manmade vagina. The extenderS of frozen Semen, cryoprotectant and the glycerol content were Studied. The reSultS Showed that the No Ⅱ extender waS the best in four grougS（p〈0.05）. The Glycerol waS the best cryoprotectant compared with Glycol and DMSO. The after freezing mobility were up to 0.45±0. 575. Abnormal Sperm poSt -- thaw waS 15.44 percent ,and intact rate Sperm acrpSome post -- thaw waS 57.82 percent; The glyceol content is 7 percent（p〈0.05）,the Sperm motility post --thaw iS the highest in four treatment groupS. The Semen motility iS 0.45±0.575 ,the abnormal Sperm post--thaw iS 15.44 percent, the intact rate Sperm acroSome poSt--thaw 57.82 percent. The best thawing method iS in 75℃ for 2 to 6S.

关 键 词：奶牛 稀释 冷冻保护剂 甘油浓度 冻粗质量 

分 类 号：S814.4[农业科学—畜牧学]