羊踯躅花瓣总RNA提取方法的比较与改进  

Comparison and Improvement of the Methods for Total RNA Isolation from the Petals of Rhododendron molle

作　　者：王燕[1] 王曼莹 李思光[1] 

机构地区：[1]南昌大学生命科学学院,江西南昌330031 [2]江西省亚热带植物资源保护与利用重点实验室,江西南昌330022

出　　处：《南昌大学学报（理科版）》2008年第1期62-65,共4页Journal of Nanchang University(Natural Science)

基　　金：江西省农业科技攻关资助项目(赣科发计字[2004]65号)

摘　　要：不同组织的RNA提取难点各异,适宜方法也不尽相同。分析、比较了热酸性酚抽提法、热硼酸法、异硫氰酸胍法和改良的异硫氰酸胍法从羊踯躅花瓣中提取总RNA的效果,经过多次反复实验发现采用改良的异硫氰酸胍法所得的总RNA质量好,纯度高,完整性很好,适用于cDNA文库的构建、Northern印迹与mRNA差异显示等后续研究工作。The appropriate methods are not always same because of the difference in isolating RNA from diverse plants and tissues. In this paper, four methods of total RNA isolation from the petals of Rhododendron molle were discussed,including method of hot phenol, hot borate, guanidinium thiocyanate and modified guanidinium thiocya- nate. The RNAs isolated with method of modified guanidinium thiocyanate were proved to have good quality, purity and integrity, and can be used in cDNA library construction, northern blotting, mRNA DDRT- PCR and so on.

关 键 词：羊踯躅花瓣 RNA提取 异硫氰酸胍法 改良 

分 类 号：Q949.772.3[生物学—植物学]