愈心痛对犬急性缺血心肌ECE、NOS基因表达的影响及铜锌-SOD免疫组织化学研究  

Effect of Yuxintong on ECE, NOS Gene Expression and Immunohistocbemical Studies on Cooper-Zinc-SOD in Ischemic Myocardium in Experimental Dosgs

作　　者：雷燕[1] 史大卓[1] 钟蓓[1] 陈可冀[1] 

机构地区：[1]中国中医研究院西苑医院,北京建工医院心内科,北京中医药大学

出　　处：《中国中西医结合杂志》1997年第10期607-610,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的：探讨益气活血复方愈心痛胶囊对缺血心肌组织内皮素转换酶（ＥＣＥ）、一氧化氮合酶（ＮＯＳ）基因表达和铜锌超氧化物歧化酶（铜锌－ＳＯＤ）免疫组织化学反应的影响，阐明愈心痛胶囊抗心肌缺血的作用机制。方法：采用结扎麻醉犬冠状动脉左前降支中段的方式，造成实验性犬急性心肌缺血病理模型，以原位杂交技术检测缺血心肌中ＥＣＥ和ＮＯＳｍＲＮＡ的表达，用免疫组织化学方法观察缺血心肌中铜锌－ＳＯＤ阳性反应程度。结果：在缺血心肌组织中，ＥＣＥｍＲＮＡ呈高表达，ＮＯＳｍＲＮＡ呈低表达，愈心痛可提高缺血心肌中ＮＯＳｍＲＮＡ表达水平，削弱ＥＣＥｍＲＮＡ的异常表达，从而保护缺血心肌；同时，缺血心肌中铜锌－ＳＯＤ免疫反应减弱，而愈心痛可显著增强缺血心肌组织中铜锌－ＳＯＤ阳性反应程度，提高ＳＯＤ活性。结论：愈心痛胶囊的上述抗心肌缺血的作用，部分说明了益气活血方药的作用机理，提示该方可能对缺血性心脏病的治疗具有潜在的临床应用价值。Objective: To explore the effect of Yuxintong (YXT) on the endothelin converting enzyme (ECE) and the nitric oxide synthase (NOS) gene expression levels and cooper-zinc-superoxide dismutase(SOD) activity. Methods:The acute myocardial ischemia models were reproduced in anesthetized dogs by ligating the left coronary artery at the middle of anterior descending branch. The ECE and NOS gene expressions were observed by in situ hybridization and the cooper-zinc-SOD was studied by immunohistochemical and image analysis. Results: It was found that the transfer of ECE mRNA was greatly increased, while that of NOS mRNA was greatly decreased in ischemic myocardium, but the YXT could enhance the expression levels of NOS mRNA and inhibit the over expression of ECE mRNA in ischemic myocardium in dog. Meanwhile, the cooper-zinc-SOD immunohistochemistry was significantly weakened in ischemic myocardium, but the YXT could evidently increase SOD activity in experimental ischemic myocardium. Conclusions: It suggested that YXT had notable protection on experimental ischemic myocardium and was definitely valuable on prevention and treatment of coronary heart disease.

关 键 词：愈心痛胶囊 心肌缺血 内皮素转换酶 

分 类 号：R259.422[医药卫生—中西医结合]