抑制性消减杂交技术在对虾基因克隆中的应用  

Application of Suppression Subtractive Hybridization in Shrimp Gene Cloning

作　　者：赵永贞[1] 蒋伟明[1] 陈秀荔[1] 李咏梅[1] 张彦明[2] 黎铭[1] 陈晓汉[1] 

机构地区：[1]广西水产研究所广西水产遗传育种与健康养殖重点实验室,广西南宁530021 [2]西北农林科技大学动物医学院,陕西杨陵712100

出　　处：《动物医学进展》2008年第4期76-80,共5页Progress In Veterinary Medicine

基　　金：广西科学研究与技术开发计划(桂科基0731034)

摘　　要：抑制性消减杂交技术是一项筛选、分离未知差异表达基因的试验方法,该技术具有快速、简便、灵敏、特异、高效,所需起始样本量较少等优点。利用该技术构建对虾组织消减cDNA文库,鉴定差异表达相关基因及其表达特性。通过对对虾差异性表达的免疫相关基因、抗病基因及其他性状基因的了解,旨在为进一步研究对虾抗病机理奠定基础,为利用抗病基因进行抗病育种提供一个有效途径,为提高对虾的经济效益提供前景。Abstract：Suppression subtractive hybridization（SSH） technology is an experimental method that sieves and separates unknown differentially expressed genes. It possess some merits of speediness, brief, conven- ience, sensitivity, speciality, effectiveness and less quantity of the original sample, and so on. SSH was used to construct subtraetive eDNA libraries by suppression subtractive hybridization, which was em- ployed to identify differentially expressed genes and their expression profiles in shrimp tissue. Research on differentially expressed immune-relevant genes, virus-resistant genes and other genes, in order to build good base through further studying antiviral mechanism in shrimp, offers an effective channel for antiviral breeding through antiviral genes,and offers a prospect for raising economic effectiveness.

关 键 词：抑制性消减杂交 对虾 差异表达基因 CDNA文库 

分 类 号：Q78[生物学—分子生物学] S945.46[农业科学—水产养殖]