丹参酮对人肝癌细胞某些表型的逆转作用  

THE REVERSING EFFECT OF TANSHINONE ON MALIGNANT PHENOTYPE OF HUMAN HEPATOCARCINOMA CELL LINE

作　　者：袁淑兰[1] 黄韧敏[1] 王修杰[1] 宋毅[1] 黄光琦[1] 

机构地区：[1]华西医科大学肿瘤研究所

出　　处：《肿瘤》1997年第5期268-270,共3页Tumor

基　　金：卫生部和CMB基金

摘　　要：目的观察丹参酮促人肝癌细胞株在体外向正常方向分化的效果。方法体外培养的人肝癌细胞（ＳＭＭＣ┐７７２１）经０．５μｇ／ｍｌ丹参酮处理４天后，作光电镜观察，ＢｒｄＵ掺入试验，ＰＣＮＡ免疫组化及ＦＣＭ检测。结果在镜下，细胞形态趋向良性分化，细胞生长明显被抑制；ＢｒｄＵ标记率和ＰＣＮＡ阳性率均明显低于对照组；流式细胞仪检测显示丹参酮处理组的细胞被阻止于Ｇ０／Ｇ１期，而Ｓ期细胞数量明显减少，ｃ┐ｍｙｃ癌基因蛋白表达降低，ｃ┐ｆｏｓ癌基因蛋白表达明显增加。结论丹参酮可诱导人肝癌细胞某些表型的逆转，可能是一种有前途的分化诱导剂。Objective:The reversing effect on malignant phenotype of cancer cell by Chinese drug Tanshinone was studied. Methods:Human hepatocarcinoma cell line (SMMC 7721)was treated in vitro with 0 5μg/ml Tanshinone for 4 days, and then test for variation in cell differentiation Results: The morphology of cancer cells was tended toward well differentiation. Cell growth was markedly inhibited BrdU incorporate assay and immuno histochemical stain of PCNA showed that the BrdU labeling rate and PCNA positive rate were lower than the controls,but no difference was found statistically compared with all trans retinoic acid(ATRA). Flow cytometry demonstrated that S phase cells decreased and G 0/G 1 phase cells increased,Expression of c myc oncogene protein decreased but the c fos oncogene protein markedly increased. Conclusions: Tanshinone could have the effect of inducing differentiation in human hepatocarcinoma cell (SMMC 7721).It may become a new prospective inducer of cell differentiation.

关 键 词：丹参酮 肝癌 逆转 

分 类 号：R273.57[医药卫生—中西医结合]