扩增香螺SSR指纹研究的试验条件的优化  

Optimization of Fingerprint on Simple Sequence Repeats(SSR) of Conch Neptunea cumingi

作　　者：隋娜[1] 侯林[1] 董长勇[1] 李妍[1] 王明昌[1] 张筠[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《水产科学》2008年第4期199-202,共4页Fisheries Science

摘　　要：利用SSR技术对香螺进行了PCR扩增,探索香螺基因组DNA的提取方法。从香螺23对近缘种引物中筛选出8对可以扩增的引物,对引物浓度、Taq酶浓度、循环次数、模板浓度等方面进行了研究,从而探索出一个适合香螺SSR分析的反应体系和反应条件并对微卫星引物通用性进行初步分析。Simple Sequence Repeats（SSR） technique was applied to amplify the genomic DNA of conch Neptunea cumingi and a suitable method was studied for DNA extraction.The conditions for SSR analysis of the conch were optimized in order to produce clear amplified fragments,such as the concentration of primer,DNA and Taq, and the reaction systems and the reaction conditions.A suitable SSR analysis and common microsatellite primers were discussed.

关 键 词：香螺 SSR 条件优化 

分 类 号：Q25[生物学—细胞生物学]