鹿鞭的微量DNA提取及序列鉴定  

Extraction and Sequencing of Trace DNA from Cornu cervi Pantotrichum

作　　者：王建云[1] 何广新[1] 付文[1] 宿兵[2] 王文[2] 张亚平[2] 陆源[2] 何牧 朱兆云 

机构地区：[1]云南省药品检验所 [2]中国科学院昆明动物研究所 [3]昆明圆通动物园 [4]云南省药材公司

出　　处：《中国中药杂志》1997年第10期579-583,共5页China Journal of Chinese Materia Medica

摘　　要：采用微量ＤＮＡ提取技术，从梅花鹿血、毛、鹿鞭、鹿茸、牛鞭、驴鞭中提取ＤＮＡ，以线粒体ＤＮＡ细胞色素ｂ通用引物Ｌ１４８４１和Ｈ１５１４９扩增约３０７ｂｐＤＮＡ片段，扩增产物纯化后采用双脱氧链终止法测定其序列。结果证明：梅花鹿毛、血和鹿鞭的ＤＮＡ序列完全一致；而所谓的“鹿茸”则与其有较大的差异。用所测序列以简约法ＰＡＵＰ３１１程序构建的分子系统树与传统分类系统相吻合。说明此方法鉴定鹿鞭是科学而准确的。The trace DNA extraction method was used to extract DNA from the blood,hair,Cornu cervi pantotrichum. hairy antler of the sika deer,Cervus nippon,and penis of Bulbalus bublis and Asinus vulgaris. A 307bp fragment of mitochondrial DNA cytochrome b gene was amplified with primers L14841 and H15149 for these extractions.The purified PCR products were sequenced by the dideoxy method.DNA sequence obtained from the Cornu cervi pantotrichum is the same as that obtained from the fresh blood and hair of the sika deer,but the sequence of the so called hairy antler is quite different,implying that it is not from the sika deer.In addition,the phylogenetic relationships among different species are congruent with the traditional known phylogeny.

关 键 词：鹿鞭 DNA 序列测定 动物药材 中药鉴定 

分 类 号：R282.740.3[医药卫生—中药学]