能量对初情期前母猪卵巢和子宫IGF-1R和EGFR mRNA表达的影响  

Effects of Dietary Energy Levels on Ovarian and Uterine Expression of IGF-1R and EGFR mRNA in Prepubertal Gilts

作　　者：李波[1] 张贺[1] 张晶[1] 孙博兴[1] 陈璐[1] 孙艳玲[1] 周虚[1] 

机构地区：[1]吉林大学畜牧兽医学院

出　　处：《农业生物技术学报》2008年第2期225-229,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30371033);国家科技支撑计划课题(No.2006BAD14B08)资助

摘　　要：采用初情期前母猪军牧1号9头,随机分成3组,分别饲喂高、中、低3个能量组饲料,预饲7日后进入实验期,维持14日,取下每头母猪卵巢和子宫并用液氮保存。结果表明,①卵巢,高能组猪胰岛素样生长因子-1受体(IGF-1R)和表皮生长因子受体(EGFR)mRNA的表达量显著高于低和中能量组(P<0.05),低能组这两种生长因子受体表达量显著低于中能组(P<0.05)。②子宫,高能组猪IGF-1R和EGFRmRNA的表达量显著高于中、低能组(P<0.05),低能组低于中能组,但差异不显著(P>0.05)。日粮能量水平显著影响初情期前母猪卵巢两种生长因子受体的表达,高能量对表达有促进作用,有利于两种生长因子作用的发挥,从而利于生殖系统的生长发育,表明IGF-1R和EGFR可能参与介导能量摄入对初情期前母猪生殖系统发育的影响。Nine prepubertal gilts （JunMu No.1） were randomly allocated to three groups （n =3） and fed with a high level digestive energy （Group H）, a low level digestive energy （Group L） and a control group digestive energy （Group M） diet for 14 d, respectively. Free access of water was provided throughout the whole research period. Ovaries and uteruses were collected after 2 weeks energy treatment, and processed for determination of the absolute quantities of insulin-like growth factor receptor （IGF-1R） and epidermal growth factor receptor（EGFR） mRNA by using real-time PCR. The ovaries and uteruses expressed significantly amount of IGF-IR and EGFR mRNA in the Group H than in the Group M and Group L（P〈0.05）. And the ovaries in the Group M expressed significantly larger amount of IGF-1R and EGFR mRNA（P〈0.05） than that in the Group L. The present study demonstrated that high energy intake markedly enhanced the ovarian and uterine expression of IGF-1R and EGFR in prepubertal gilts, whereas, insufficient energy intake markedly inhibited such expressions. The result suggests that IGF-1R and EGFR may be involved in mediating the effects of energy intake on the development of reproductive system in prepubertal gilts.

关 键 词：日粮能量水平 表皮生长因子受体 胰岛素样生长因子-1受体 初情期前母猪 

分 类 号：S188[农业科学—农业基础科学]