用0.25mL细管冷冻保存猪精液  

Cryopreservation of Boar Semen Using 0.25 mL Straws

作　　者：高俊锋[1] 郑筱峰[1] 葛立军[1] 卢庆[1] 芮荣[1] 

机构地区：[1]南京农业大学动物医学院,南京210095

出　　处：《农业生物技术学报》2008年第2期264-269,共6页Journal of Agricultural Biotechnology

摘　　要：研究旨在建立优化的猪精液冷冻保存方法。实验表明,采用ZORLESCO(ZO)液对猪精液进行预稀释,室温平衡1h,加入冷冻Ⅰ液后于5℃水浴平衡1.5h,再加入冷冻Ⅱ液平衡2h后装入0.25mL细管,液氮面上3cm处平衡10min投入液氮,采用37℃水浴解冻30s,可获得最佳冷冻效果。解冻后的精子活力平均为0.58±0.03,质膜完整率为(63.2±1.2)%,顶体完整率为(51.4±2.6)%;精子畸形率最低,仅为(14.0±3.0)%。The purpose of this study is to establish an optimal protocol to cryopreserve boar semen. The results showed that the optimal procedures should be operated as follows. Firstly, boar semen were pre-diluted with ZORLESCO（ZO） solution and pre-equilibrated at room temperature for 1 h. After adding the extender Ⅰ, spermatozoa were equilibrated at 5 ℃ for 1.5 h, then equal volume of the extenderⅡ was added, holding 2 h for equilibration. Resulting spermatozoa were loaded into 0.25 mL straws and equilibrated for 10 min at 3 cm above the surface of liquid nitrogen （LN） , and submerged into LN promptly. When thawing, straws were put into water bath at 37 ℃ for 30 s. The above procedures yielded the highest post-thaw motility of 0.58±0.03 and the plasma integrity of （63.2±1.2）%, together with the normal acrosome was （51.4±2.6）%. The abnormality of spermatozoa after freezing was the lowest at （14.0±3.0）%.

关 键 词：猪 精液 冷冻保存 

分 类 号：S188[农业科学—农业基础科学]