参附注射液对大鼠移植肝脏缺血再灌注损伤的保护作用  

Investigation of protective role of Shenfu injection in ischemia-reperfusion injury of transplanted livers in rats

作　　者：张义彬[1] 涂兵[1] 龚建平[1] 刘长安[1] 陈蓁臻[1] 郝荣涛[1] 

机构地区：[1]重庆医科大学附属第二医院肝胆外科,重庆400016

出　　处：《重庆医科大学学报》2008年第4期428-431,445,共5页Journal of Chongqing Medical University

摘　　要：目的:研究参附注射液(Shenfu injection,SF)对大鼠移植肝脏在缺血再灌注损伤中的保护作用及其具体机制。方法:选用体重200～230g的雄性SD大鼠48只,随机分为对照组和SF组,进行肝脏移植。分别于肝移植完成后2、4、6h测定大鼠的胆汁分泌量,血清丙氨酸转移酶(ALT)和肿瘤坏死因子-α(TNF-α)水平,肝脏组织中核因子(NF-κB)的表达,以及肝脏组织的形态学观察(光镜、电镜)。结果:在各个时间点,SF组的胆汁分泌量明显高于对照组(P<0.05),ALT、TNF-α含量明显低于对照组(P<0.05),肝脏组织中NF-κB的表达较对照组明显减弱(P<0.05)。光镜和电镜下,SF组各时间点肝组织的损伤均较对照组明显减轻。结论:SF能明显减轻移植后肝脏结构和功能上的破坏,提高机体耐受缺血再灌注损伤的能力。Objective：To investigate the protective role of Shenfu injection（SF） in ischemia-reperfusion injury of transplanted livers in rats. Methods：48 male SD rats,weighted 200-230g,were selected and divided into control group and SF group at random. All of them were performed liver transplantation. 2h,4h and 6h after the transplantation,they were analyzed for level of biliation,semm alanine aminotransferase,tumor necrosis factor-α（TNF-α ）,NF-κB expression in hepatic tissue and the morphological observation of hepatic tissue under light/electron microscope. Results：At each phase,biliation in SF group was significantly higher than in control group（P〈0.05）;serum levels of ALT and TNF-α ,NF-κ B expression in hepatic tissue of SF group were significantly lower than in control group（P〈0.05）. At each phase,hepatic injury in SF group was significantly lighter than in control group under light/electron microscope. Conclusion：Shenfu injection can significantly palliate the injury of liver structure and function after liver transplantation,and enhance the tolerance of organism to ischemia-reperfusion injury.

关 键 词：参附注射液 肝脏移植 缺血再灌注损伤 

分 类 号：R256.49[医药卫生—中医内科学]