大黄对烫伤大鼠胃肠激素变化的影响  

Effect of rheum on gastrointestinal hormones in rats with severe scald injury

作　　者：郭杏[1] 谭美云[2] 熊爱兵[1] 郭力[1] 

机构地区：[1]四川泸州医学院附属医院整形烧伤科,646000 [2]四川泸州医学院附属医院骨科,646000

出　　处：《中华烧伤杂志》2008年第2期111-113,共3页Chinese Journal of Burns

基　　金：四川省科技厅重点科技自筹项目（05JY029-143）;四川省教育厅自然科学科研项目（2003C011）

摘　　要：目的了解严重烫伤大鼠早期血浆中胃肠激素的变化及大黄对胃肠激素水平的影响。方法88只Wistar大鼠随机分为正常对照组（8只）、烫伤对照组（40只，Ⅲ度烫伤后管饲蒸馏水）、烫伤治疗组（40只，Ⅲ度烫伤后管饲大黄水提液）。于烫伤后6、12、24、48、72h取大鼠腹腔静脉血，用放射免疫法检测血浆胃动素（MTL）、P物质、血管活性肽（VIP）、生长抑素（SS）的含量。结果（1）正常对照组大鼠MTL和P物质分别为（198±28）、（61±10）ng／L；烫伤对照组明显下降，最低值分别为（110±15）、（30±5）ng／L，而后缓慢上升，72h达高峰，但仍未恢复正常（P〈0．05）；烫伤治疗组MTL和P物质下降趋势明显变缓，各时相点均高于烫伤对照组，伤后48h已达正常水平，72h分别为（232±32）、（73±11）ng／L，明显高于正常对照组（P〈0，05）。（2）正常对照组大鼠VIP和ss分别为（35±6）、（30±5）ng／L；烫伤对照组呈明显上升趋势，伤后6h分别为（70±12）、（49±9）ng／L（P〈0．01），而后缓慢下降但72h时仍高于正常水平（P〈0．05）；烫伤治疗组VIP和SS上升趋势幅度较小，各时相点均低于烫伤对照组，48h恢复至正常水平；VIP达最高值的时间推迟至伤后12h。结论大黄对严重烫伤大鼠早期血浆中胃肠激素的分泌和释放有明显的调控作用。Objective To observe the changes in motilin（MTL） ,substance P（SP） ,vasoactive intestinal peptide（VIP） and somatostatin（SS） in plasma of rats with severe scald injury at early stage and the effect of rheum on their changes. Methods Eighty-eight Wistar rats were randomly divided into normal control group （ NC, n = 8 ） , scald group （ S, garage of distilled water after full-thickness scald, n = 40 ） , therapeutic group（ T, garage of rheum solution after full-thickness scald, n = 40）. The blood samples were harvested from inferior vena cava at 6,12, 24, 48,72 post scald hours （PSH） to determine the levels of MTL, SS, SP and VIP with radioimmunoassay. Results （ 1 ） The levels of MTL and SP were （ 198 ± 28 ） , （ 61 ± 10） ng/L,respectively, in NC group. The levels of MTL and SP in S group reached their minimum values [ （ 110 ± 15）, （30 ± 5）ng/L, respectively] at 6 PSH , then ascended slowly , peaked at 72 PSH but still lower than those in NC group （ P 〈 0.05 ）. The levels of MTL and SP slowly descended in T group, reached normal levels at 48 PSH , and obviously higher than those in NC group at 72 PSH [ （ 232 ± 32） , （73 ± 11 ）ng/ L,respectively, P 〈0.05] ,which were higher than those in S group at 6 -72 PSH. （2） The levels of VIP and SS were （35 ± 6） , （30 ± 5 ）ng/L ,respectively ,in NC group. The levels of VIP and SS in S group were （70 ± 12） , （49 ± 9）ng/L at 6 PSH, which were obviously higher than those in NC group（ P 〈 0.01 ） ,then descended slowly, but still higher than normal level at 72 PSH （ P 〈0.05 ）. The levels of VIP and SS in T group ascended slowly,reached the normal level at 48 PSH, which were lower than those in S group at each time points, and VIP reached peak value at 12 PSH. Conclusion Rheum may regulate secretion and release of gastrointestinal hormones to plasma in rats with severe scald injury at early stage.

关 键 词：烧伤 胃肠激素类 大黄属 

分 类 号：R285.5[医药卫生—中药学]