Effect of 80.55 MeV//u^(12)C^(6+) Ions on Radiosensitivity and Cell Cycle of Human Hepatoma Cell Lines  

作　　者：魏巍 李文建 郭传玲 荆西刚 金晓东 苏旭 

机构地区：[1]Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China [2]Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China [3]National Institute for Radiological Protection, China CDC, Beijing 100088, China

出　　处：《Plasma Science and Technology》2008年第2期245-249,共5页等离子体科学和技术（英文版）

基　　金：the Ministry of Science and Technology,China(No.2003CCB00200);National Natural Science Foundation of China(No.10335050)

摘　　要：In this paper, the relationship between radiosensitivity, cell cycle alteration and the change of apoptosis in different human hepatoma cell lines irradiated by heavy ions were studied with the aim of building up the base data for clinical therapy. Exponentially growing hepatoma cell lines were irradiated by 80.55 MeV/u12C6＋ ions at a dose of 0 Gy, 0.5 Gy, 1 Gy, 2 Gy, 4 Gy and 8 Gy. The radiosensitivity was assessed by means of the colony-forming assay. The DNA content, the percentage of each cell-cycle phase and the apoptosis rate were obtained with flow cytometry methods. After the irradiation, the SF2 （survival fraction at 2 gray） of SMMC-7721 cells were evidently lower than that of HepG2 cells. The S phase arrest, G2/M phase arrest delay and the apoptosis in the two hepatoma cell lines varied with the increase of the dose and repair time. The heavy ions could obviously kill the human hepatoma cell lines. Compared to HepG2 cells, SMMC-7721 cells were more radiosensitive to 12C^6＋ ions.In this paper, the relationship between radiosensitivity, cell cycle alteration and the change of apoptosis in different human hepatoma cell lines irradiated by heavy ions were studied with the aim of building up the base data for clinical therapy. Exponentially growing hepatoma cell lines were irradiated by 80.55 MeV/u12C6＋ ions at a dose of 0 Gy, 0.5 Gy, 1 Gy, 2 Gy, 4 Gy and 8 Gy. The radiosensitivity was assessed by means of the colony-forming assay. The DNA content, the percentage of each cell-cycle phase and the apoptosis rate were obtained with flow cytometry methods. After the irradiation, the SF2 （survival fraction at 2 gray） of SMMC-7721 cells were evidently lower than that of HepG2 cells. The S phase arrest, G2/M phase arrest delay and the apoptosis in the two hepatoma cell lines varied with the increase of the dose and repair time. The heavy ions could obviously kill the human hepatoma cell lines. Compared to HepG2 cells, SMMC-7721 cells were more radiosensitive to 12C^6＋ ions.

关 键 词：heavy ions human hepatoma cell lines RADIOSENSITIVITY cell cycle cell apoptosis 

分 类 号：R735.7[医药卫生—肿瘤]