大鼠局灶性脑缺血后磷酸化的细胞周期相关蛋白的表达  

EXPRESSION OF PHOSPHORYLATED CELL CYCLE-RELATED PROTEINS AFTER FOCAL CEREBRAL ISCHEMIA IN RATS

作　　者：解翠红[1] 王伟[2] 喻志源[2] 杜晓萍[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊内科,武汉430030 [2]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2008年第1期1-5,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金重点项目(30230140)

摘　　要：目的探讨细胞周期对于大鼠局灶性脑缺血后神经元的影响。方法采用MCAO方法制作大鼠局灶性脑缺血模型,应用免疫荧光技术观察缺血后1d、3d、7d、14d大鼠缺血侧病灶周围神经元中磷酸化细胞周期蛋白CDK2、CDC2及磷酸化Rb的表达。结果与正常对照组相比,缺血后1d、3d组磷酸化CDK2和磷酸化Rb的表达量明显增加(P<0.05)。缺血后7d、14d组磷酸化CDK2和磷酸化Rb的表达量无增加。磷酸化CDC2在正常组及缺血组均无明显表达。结论大鼠局灶性脑缺血后早期部分神经元再次进入细胞周期,提示细胞周期调控参与了大鼠局灶性脑缺血后神经元的凋亡。Objective To observe the expression of phosphorylated cell cycle-related proteins and the activity of the retinoblastoma tumor suppressor protein （Rb） after focal cerebral ischemia in ratsi Methods We used immunofluorescence staining for anti-phospho-CDK2, anti-phospho-CDC2, anti-phospho-Rb and anti-NeuN antibodies to determine their expression in the cortex of Wistar rats 1 d, 3d, 7d and 14d after focal cerebral ischemia. Results The expression of phospho-CDK2 and phospho-Rb increased in the neurons near the infarction in the ld and 3d groups when compared with that of the control group. In the 7d and 14d groups, there was no significant change of expression. In all groups, there were no expression of phospho-CDC2. Conclusion The expression of phospho- CDK2 and phospho-Rb suggests that differentiated neurons can re-enter the cell cycle, and more neurons enter the cell cycle in the early stage after ischemia. Re-entering of differential neurons into the cell cycle might participate in apoptosis.

关 键 词：脑缺血 磷酸化周期素依赖性激酶 磷酸化Rh NEUN 

分 类 号：R322.811[医药卫生—人体解剖和组织胚胎学]