丙酮酸氧化酶方法在血清ALT检测中的应用  

The Application of Pyruvate Oxidase Method in Detecting Serum ALT

作　　者：孙贵杰 杨槐波 

机构地区：[1]通辽市中心血站,内蒙古通辽028000

出　　处：《内蒙古民族大学学报（自然科学版）》2008年第2期210-211,共2页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：目的:探讨丙酮酸氧化酶法在测定血清丙氨酸氨基转移酶中的作用.方法:利用丙酮酸氧化酶氧化丙酮酸生成过氧化氢,过氧化氢与2,4,6三溴-3-羟基苯甲酸和4-氨基比林在过氧化物酶作用下生成红色醌类化合物的原理.结果:该方法线性范围达200 U/L,与紫外酶偶联速率法比较,相关系数r=0.989,y=1.075x+1.031,n=80.结论:该方法具有线性范围大、灵敏、操作简便、快速、结果准确、重复性好、抗干扰能力强等优点,适合血站大批量标本的检测.Objective： To investigate the application of pyruvate oxidase method to detect serum ALT with enzyme labeling meter. Methods： On the basis of the principle of pyruvate oxidase oxygenizing pyruvate to form hydrogen dioxide, and hydrogen dioxide combined with TBHBA and 4 - AAP to form red quinone compound catalyzed by peroxydase. Results： The linear range of this method amounted to 200U/L, and coefficient correlation of this method was r = 0. 989, y = 1. 075x ＋ 1. 031 （n = 80）compared with UV method of coupling method. Conclusion： The method possesses the merits of wide linear range, sensitiveness, simple manipulation, high - speed, accuracy, good reproducibility and great ability of antiturbulence. It is suitable to detect the enormous blood samples in blood bank.

关 键 词：丙酮酸氧化酶 丙氨酸氨基转移酶 ALT测定 丙酮酸氧化酶法 

分 类 号：R446.11[医药卫生—诊断学]