人参皂苷Rg_2对拟血管性痴呆大鼠学习记忆的影响  

Effect of ginsenoside-Rg_2 on learning and memory of vascular dementia rats

作　　者：张荔[1] 潘志远[1] 金毅[2] 龙超良[1] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所 [2]青岛大学医学院

出　　处：《中国临床药理学与治疗学》2008年第3期276-282,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究人参皂苷Rg2对拟血管性痴呆大鼠学习记忆的影响,并探讨其与谷氨酸离子型受体基因表达的关系。方法:双侧颈总动脉永久性结扎制备拟血管性痴呆大鼠模型,人参皂苷Rg2治疗后,检测大鼠自发性活动变化并用水迷宫法检测大鼠学习记忆变化;采用RT-PCR方法检测海马NR1、NR2A、NR2B和GluR2基因表达的变化。结果:拟血管性痴呆大鼠自发性活动显著减少,学习记忆能力明显下降,表现为信息游泳时间和选择游泳时间延长;人参皂苷Rg22.5、5.0、10.0mg/kg能剂量依赖性地增加大鼠自发性活动,缩短信息游泳时间和选择游泳时间,提高选择正确率,显著改善大鼠学习记忆障碍。拟血管性痴呆大鼠海马谷氨酸离子型受体亚基NR1mRNA表达显著增加,而NR2A、NR2B和GluR2mRNA的表达均显著降低,人参皂苷Rg2能逆转此变化。结论:人参皂苷Rg2能显著改善大鼠学习记忆障碍,机制可能与其有效调节脑内谷氨酸受体亚基基因的表达有关。AIM： To investigate the effect of ginsenoside-Rg2 on learning and memory of vascular dementia rats and its underlying mechanisms, especially the expression of NMDA/AMPA receptors. METHODS： Vascular dementia （VD） of rat was induced by permanent occlusion of bilateral common carotid arteries（2OV）. The rats were given different doses of ginsenoside-Rg2 （2. 5, 5.0, 10.0 mg/kg） as while as operation. The spontaneous movement and the delayed non-matching-to-position task（DNMPT） in the water maze were used to examine the behavior changes on learning and memory; the mRNA of four different subtypes of NMDA/AMPA receptors, including NR1, NR2A, NR2B and GluR2 in hippocampus were determined by the RT-PCR method. RESULTS： The spontaneous movement and spatial learning and memory of VD rats were all decreased. After ginsenoside-Rg2 administration, the spontaneous movement and the spatial learning and memory were improved; the latency of information swim （IS） and choice swim （CS） decreased, and choice accuration in choice swim increased. The expression of NR1 mRNA was enhanced while NR2A, NR2B and GluR2 were attenuated in hippocampus of VD rats. These pathological changes could be reversed by treatment with ginsenoside-Rg2. CONCLUSION： Ginsenoside-Rg2 can patently improve the spatial learning and memory of VD rats. The mechanism may be related to the regulation of ginsenoside-Rg2 on the expression of NMDA/AMPA receptors in hippocampus.

关 键 词：血管性痴呆 人参皂苷RG2 水迷宫 学习记忆 

分 类 号：R965.2[医药卫生—药理学]