株多重耐药肺炎克雷伯菌耐药基因和整合子、转座子遗传标记研究  

A clinical study of the multi-drug resistant Klebsiella Pneumoniae and its related resistant genes including Integrons and Transposons

作　　者：王继东[1] 盛光杰[1] 钱小毛[2] 糜祖煌[3] 周丽珍[1] 

机构地区：[1]江苏省无锡市人民医院,江苏无锡214023 [2]浙江省绍兴第二医院,浙江绍兴312000 [3]江苏省无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《世界感染杂志》2008年第2期94-97,共4页World Journal of Infection

摘　　要：目的了解多重耐药肺炎克雷伯菌的耐药基因存在状况和遗传学背景。方法聚合酶链反应法对多重耐药的肺炎克雷伯菌进行β-内酰胺酶基因、氨基糖苷类修饰酶基因、质粒AmpC酶基因、qacE△1-sull耐消毒剂和磺胺基因、整合子遗传标记（整合酶基因）、Tn21／Tn501转座子遗传标记（汞离子还原酶基因）检测。结果TEM、SHV型β-内酰胺酶基因，DHA型质粒AmpC酶基因，aac（6’）-I型氨基糖苷类修饰酶基因，qacE△1-sull耐消毒剂和磺胺基因，整合子遗传标记（intIl整合酶基因），Tn21／Tn501转座子遗传标记（merA汞离子还原酶基因）检测阳性。结论多重耐药肺炎克雷伯菌存在多种耐药基因和I类整合子、Tn21／Tn501转座子。Objective To survey the presence and genetic background of drug resistant genes in multi-drug resistant Klebsiella Pneumoniae. Methods Amplify the following genes： beta-lactamase gene; aminoglycoside resistance gene; plasmid-mediated AmpC enzyme gene; qacE△1 gene and sul I gene which resist various disinfectant and sulfonamides; Integrons and Tn21/Tn501 mercury transposons by PCR and detect their presence in multi-drug resistant Klebsiella Pneumoniae. Results Multi-drug resistant Klebsiella Pneumoniae is detected positive for the following genes： TEM and SHV type of beta-lactamase gene; DHA type of plasmid-mediated AmpC enzyme gene; aminoglycoside resistance genes aac（6＇）-I; qacE△1 gene and sul I gene which resist various disinfectant and sulfonamides; Integron I and Tn21/Tn501 mercury transposon （mercury reductase （merA） like genes）. Conclusions Multi-drug resistant Klebsiella Pneumoniae is detected positive for an array of drug resistance genes, Integron I and Tn21/Tn501 transposons

关 键 词：肺炎克雷伯菌 多重耐药 基因 整合子 转座子 

分 类 号：R978.11[医药卫生—药品] R378.911[医药卫生—药学]