大肠埃希菌、肺炎克雷伯菌连续分离株β-内酰胺酶基因研究  

Study on the β-lactamase genes of continuously isolated Escherichia coli and Klebsiella pneumoniae strains

作　　者：糜祖煌[1] 钱小毛[2] 黄支密[3] 

机构地区：[1]无锡市克隆遗传技术研究所,江苏无锡214026 [2]绍兴第二人民医院,浙江绍兴312000 [3]解放军98医院,浙江湖州313000

出　　处：《世界感染杂志》2008年第2期101-105,共5页World Journal of Infection

摘　　要：目的了解大肠埃希菌、肺炎克雷伯菌连续分离株β-内酰胺酶基因存在状况。方法采用PCR检测浙江地区二家医院大肠埃希菌、肺炎克雷伯菌连续分离株21种β-内酰胺酶基因。结果检出TEM、SHV、LEN、CTX-M-1 cluster、CTX-M-9 cluster、OXA-1 cluster、OXA-10 cluster、LAP、DHA、ACT-1等β-内酰胺酶基因，并在国内外首次从大肠埃希菌、肺炎克雷伯菌中发现LAPβ-内酰胺酶基因。结论浙江地区二家医院大肠埃希菌、肺炎克雷伯菌连续分离株β-内酰胺酶基因携带率高。存在新的β-内酰胺酶基因（LAP）。Objective In order to understand β-lactamase gene status in continuously isolated Escherichia coli and Klebsiella pneumoniae strains. Methods 21 kinds of β-lactamase genes were detected by PCR in two Zhejiang hospitals. Results There were TEM, SHV, LEN, CTX-M-1 cluster, CTX-M-9 cluster, OXA-1 cluster, OXA-10cluster, LAP, DHA and ACT-1 genes in two Zhejiang hospitals. The LAP β-lactamase gene is found from Escherichia coli and Klebsiella pneumoniae in world for the first time. Conclusions The β-lactamase gene carrying rate is high in continuously isolated Escherichia coli and Klebsiella pneumoniae strains in two Zhejiang hospitals. There is new β-lactamase gene （LAP）.

关 键 词：大肠埃希菌 肺炎克雷伯菌 Β-内酰胺酶 

分 类 号：R378.2[医药卫生—病原生物学] R378.996[医药卫生—基础医学]