高原牦牛乳铁蛋白素基因克隆及序列分析  

Clone and Sequence Analyse of the Gaoyuan Yak's Lfcin Gene

作　　者：裴杰[1] 阎萍[1] 姬国红[2] 冯瑞林[1] 梁春年[1] 郭宪[1] 曾玉峰[1] 包鹏甲[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [2]甘肃农业大学草业学院,兰州730070

出　　处：《中国畜牧兽医》2008年第4期37-41,共5页China Animal Husbandry & Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF070105)

摘　　要：利用PCR技术从高原牦牛基因组DNA中获得了乳铁蛋白素(lactoferricin,Lfcin)基因序列;将Lfcin基因连接于pGEM-T easy载体,送至生物公司测序;将高原牦牛与奶牛的Lfcin基因序列进行比对;同时,对牦牛、奶牛、人、小鼠等物种的Lfcin蛋白序列进行分析。结果表明:克隆获得了含高原环湖牦牛LF(lactoferrin)第2外显子的DNA序列,共778 bp,其中Lfcin基因编码区长75 bp,编码25个氨基酸;序列分析显示,克隆获得的牦牛DNA序列与奶牛这一序列存在9个碱基的变异;牦牛和奶牛的Lfcin蛋白质序列完全相同,各物种Lfcin蛋白具有较高的同源性。Using PCR technology, the lactoferricin gene was obtained from genome of Gaoyuan yaks The Lfcin gene was cloned to pGEM-T easy vector, and the product was sequenced by bio-company, Gaoyuan yak and cow＇s Lfcin sequences were aligned; Yak, cow, human, mouse＇s Lfcin protein sequences were analysed. As a result, the DNA sequence that contains the LF gene second exon of Gaoyuan yak was cloned, constituted by 778 bp, Lfcin coding region that code 25 amino acids being 75 bp lengths According sequence analyse, there are 9 different bases between cow and yak＇s sequence; Yak and cow＇s Lfcin gene code same protein sequence and there are high homology in different species.

关 键 词：牦牛 乳铁蛋白素基因 克隆 

分 类 号：Q513.2[生物学—生物化学]