两种不同方法处理牛分离精子对体外受精效果的影响  

Effects of Bovine Flow Cytometrically-sorted Sperm with Two Different Treatments in Vitro Fertilization

作　　者：江明生[1] 陆阳清[1] 胡传活[1] 张明[1] 卢晟盛[1] 李林[1] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所,广西亚热带生物资源保护利用重点实验室,南宁530005

出　　处：《中国畜牧兽医》2008年第4期56-58,共3页China Animal Husbandry & Veterinary Medicine

基　　金：广西科技攻关项目(桂科攻0537001-1);广西大学科技研究重点项目(20052D05);广西创新能力建设项目(桂科能0630006-5B)

摘　　要：本试验旨在探讨采用2种不同方法处理牛分离精子对精子活力、浓度及体外受精(IVF)效果的影响。将体外培养成熟卵母细胞随机分为2组,与分别用洗涤法和Percoll法处理牛分离精子进行IVF,并观察处理后各组的精子活率、浓度和IVF后囊胚发育率。结果显示,洗涤法和Percoll法处理后精子活力和IVF后的囊胚发育率均无显著差异,但采用洗涤法处理后分离精子浓度极显著高于Percoll法,可显著提高分离精子利用效率,且方法简便、经济,应优先选择使用。The objective of this study was to investigate the effect of bovine flow cytometrically-sorted sperm after two treatments. Bovine oocytes were randomly divided into two groups and matured and inseminated in vitro with washing or Percoil treatment. Sperm survival rate and density were recorded following treatments. The results showed that： there was no significant difference in sperm survival and blastocyst rates between the two treatment groups, but washing treatment resulted with higher sperm density compared to percoll treatment; sperm density with washing treatment was significantly higher than that with Percoll treatment （P〈0.01）. The present study demonstrated that the efficiency of utilization of bovine flow cytometrically-sorted sperm can be improved with washing treatment as it can obtain higher sperm denesity.

关 键 词：分离精子 体外受精 洗涤法 Percoll法 

分 类 号：S814.4[农业科学—畜牧学]