结晶紫共振瑞利散射法测定七叶皂苷钠  

Resonance Rayleigh Scattering Method for the Determination of Sodium Aescinate with Crystal Violet

作　　者：周娟[1] 王祥洪[1] 

机构地区：[1]长江师范学院化学及环境科学系,重庆408100

出　　处：《重庆师范大学学报（自然科学版）》2008年第2期71-73,77,共4页Journal of Chongqing Normal University:Natural Science

基　　金：重庆市教委科研项目(No.KJ051305)

摘　　要：在pH5．0的BR缓冲溶液中，七叶皂苷钠（SA）阴离子与结晶紫（CV）阳离子反应，形成离子缔合物，此时将引起共振瑞利散射光谱RRS急剧增强并产生新的RRS光谱，结合产物的最大散射波长位于342nm，七叶皂苷钠浓度在0-20μg·mL^-1时与散射强度呈直线关系；研究了适宜的反应条件和影响因素，考察了共存物质的影响，测得七叶皂苷钠的检出限为19ng·mL^-1；实验表明结晶紫共振瑞利散射法选择性好，可用于片剂中七叶皂苷钠的测定。In pH 5.0 Britton-Robinson（BR） buffer solution, Sodium Aescinate （SA） can react with Crystal Violet （CV） cationic to form ion association complex, resulting in the great enhancement of resonance Rayleigh scattering（RRS） and the new RRS spectra appeares. The maximum RRS peak locates at 342 nm . There is a linear relationship between the enhanced RRS intensity and the Sodium Aescinate concentration in the range of 0 -20 mg · mL^-1 , The method has higher sensitivity. The detection limit for SA is 19 ng · mL^- 1. The optimum conditions are investigated, The effect of the acidity on AI shows that the optimum pH is in 5.0 and the appropriate amount is 3.5 ml ; The effect of the dye amount on △I shows that the optimum amount is 3.0 ml. The effects of various foreign substances such as talc powder, lactose, glucose, starch, dextrin, protein and some metal ions on the determination of SA are investigated. The results show that the method has good selectivity, and it has been applied to the determination of trace SA in tablet samples with satisfactory result. The method has higher sensitivity than that of the reported HPLC. So the method can be used in the determina- tion of trace amount of SA.

关 键 词：七叶皂苷钠 结晶紫 共振瑞利散射 

分 类 号：O657.3[理学—分析化学]