抗非特异性蛋白吸附的聚乙二醇接枝聚乳酸对成骨细胞黏附、生长和分化的影响  

Influence of poly(ethylene glycol) graft poly(D,L-lactic acid) with protein-resistant property on the adhesion, proliferation and differentiation of osteoblasts

作　　者：糜丽[1] 潘君[1] 赵明媚[1] 刘颖[1] 王彬[1] 王远亮[1] 

机构地区：[1]重庆大学生物工程学院生物材料与仿生工程研究中心,重庆市400044

出　　处：《中国组织工程研究与临床康复》2008年第10期1843-1846,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30300084);"111"计划"生物力学与组织修复工程学科创新引智基地"资助项目~~

摘　　要：目的:在前期成功合成聚乙二醇接枝的聚乳酸材料[poly(ethyleneglycol)graftpoly(D,L-lacticacid),PPLA]的基础上,以聚乳酸为对照,进一步考察该材料的细胞相容性。方法:实验于2006-10在重庆大学生物材料与仿生工程实验室进行。①以FITC荧光标记牛血清白蛋白检测聚乳酸及自制的PPLA材料的抗非特异性蛋白吸附性能。②在体外聚乳酸和PPLA聚合物膜上培养Wistar新生鼠成骨细胞。采用四甲基偶氮唑盐比色法测试细胞黏附和增殖情况,并通过检测碱性磷酸酶活性评价细胞分化程度。结果:①与聚乳酸相比,PPLA对牛血清白蛋白的吸附明显降低,仅为聚乳酸的37.3%。②与聚乳酸相比,在接种成骨细胞后,PPLA材料上的细胞黏附量更多,细胞数量更高;经过8d的培养后,PPLA材料上成骨细胞的碱性磷酸酶活性高于聚乳酸。结论:与聚乳酸相比,PPLA材料具有更强的抗非特异性蛋白吸附作用,并能显著地促进细胞黏附、生长及分化,具有良好细胞相容性。AIM： The poly（ethylene glycol） graft poly（D,L-lactic acid） （PPLA） has been synthesized, and this study will detect its cell compatibility compared with polylactic acid （PLA）. METHODS： The experiment was carried out in the laboratory of Biomaterials and Bionics, Chongqing University in October 2006. ①The anti-non-specific protein absorption properties of PLA and PPLA polymer were tested using FITC labeled bovine serum albumin.②Osteoblasts of Wistar neonatal rats were cultured on the PLA and PPLA polymer in vitro. The adhesion and proliferation of osteoblasts on the films of polymers were tested using 3-（4, 5-dimethylthiazal-2yl） 2, 5-diphenyltetrazolium bromide assay. The alkaline phosphatase activity was tested to evaluate the cell differentiation. RESULTS： ①Compared with PLA, the poly（ethylene glycol） graft PPLA showed an obviously decreasing absorption of bovine serum albumin, accounting for 37.3% of PLA.②Compared with PLA, the adhesion and proliferation of osteoblasts were improved after seeding on PPLA material; Alkaline phosphatase activity of osteoblasts was also advanced on PPLA material after 8 days. CONCLUSION： Compared with PLA, PPLA reveals a prominent property of non-specific protein absorption, it can significantly improve the adhesion, proliferation and differentiation of osteoblasts, as well as representing good cell compatibility.

关 键 词：聚乳酸 聚乙二醇 细胞相容性 聚乙二醇接枝聚乳酸 生物材料 

分 类 号：R318[医药卫生—生物医学工程]