构建自体骨碎末移植材料修复骨缺损的实验模型(英文)  

Repairing a bone defect model by using autogenous bone powder and implant materials

作　　者：王奕[1] 马国武[1] 

机构地区：[1]大连医科大学口腔医学院,辽宁省大连市116023

出　　处：《中国组织工程研究与临床康复》2008年第10期1987-1990,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：学术背景:自体骨移植取材常需要开辟第二术区或在种植体周围取骨,额外增加创伤和感染机会,所以对局部小范围的骨质欠缺,可以考虑回收和利用预备种植体切除的自体骨末。目的:建立恢复种植体周围骨缺损的自体骨碎末骨移植材料的实验模型,观察材料与宿主的生物相容性反应。设计:单一样本观察。单位:大连医科大学口腔医学院。材料:实验于 2005-08/2006-04 在大连医科大学动物实验基地完成。实验动物为 5 只健康杂交家犬;种植体钛钉和 Bio-Oss 骨移植材料均由西安中邦钛生物材料有限公司设计制造并提供。方法:拔除家犬下颌第 1,2,3 前臼齿,3 个月后行种植术。预备种植体窝,每只犬左右两侧各预备 4 个,共 40 个。在每个种植窝内,各植入种植体钛钉 1 枚,共 40 枚。用种植转孔时收集的自体骨碎末、Bio-Oss 骨移植材料及两者 1∶1 混合骨碎末恢复种植体颊侧单壁人为骨缺损,以未植骨作空白对照。主要观察指标:①种植术后第 9 周时观察各组骨量的恢复情况、X 射线片观察牙槽骨高度、骨小梁致密度及骨整合情况。②应用亚甲基蓝- 碱性品红法观察组织学变化。结果:5 只家犬钛钉无脱落,均纳入结果分析。①一般情况及骨缺损量:种植术后 9 周,创口愈合均良好,钛钉稳定,总存留率为 100%。植入自体骨碎末的骨缺损量小于空白对照组(P < 0.01);植入混合骨碎末的平均骨缺损量最小,说明恢复最佳。②骨量的恢复情况:X 射线片显示 40 颗钛钉外周均与骨组织紧密接触,愈合良好。③材料与宿主的组织相容性:低倍镜下见所有钛钉均被周围淡红色的致密骨组织紧密包绕,种植体与骨组织间无蓝色的软组织,产生了直接骨结合界面。结论:家犬建立自体骨碎末移植材料恢复种植体周围骨缺损的实验模型效果理想,材料与宿主间生物相容性好。BACKGROUND： Sampling the autogenous bone always needs to develop the second operational area or harvest surrounding the implant, which will increase the risk of trauma and infection. Therefore autogenous bone powder may be taken into consideration to reclaim and use for the treatment of local bone defect. OBJECTIVE： To construct experimental models of repairing bone defect by autogenous bone powder and implant material, and observe the biocompatibility between materials and host. DESIGN： Single sample observation. SETTING： College of Stomatology, Dalian Medical University. MATERIALS： The experiment was performed at the Animal Experimental Center of Dalian Medical University from August 2005 to April 2006. Five healthy hybrid dogs were used in this study. Titanium nail and Bio-Oss bone implant materials were designed and produced by Xi＇an Zhongbang Titanium Biological Materials Co., Ltd. METHODS： The first, second and third premolars of dogs were uprooted and received implantation 3 months later. Four implant sockets were prepared at left and right sides respectively of each dog, totally 40 sockets. One titanium nail was implanted into each socket, totally 40 nails. Autogenous bone powder collected when drilling hole, Bio-Oss implant materials and their mixture at 1 ： 1 were implanted in artificial bone defect at bucca side, respectively, while non-implanted bone was served as blank control. MAIN OUTCOME MEASURES： ①At week 9, recovery of bone mass was recorded. Height of alveolar bone, density of bone trabecula and osseointegration were observed by X-ray film.②Histological change was determined by methylene blue-basic fuchsin method. RESULTS： No titanium nail fell off, so five dogs were all involved in the result analysis.①General condition and bone defect： At week 9 of implantation, wound was well healed, and the titanium nail was stable with total reserved rate of 100%. Compared with blank control, the bone defect mass was less in bones implanted with autogenous bone powder （P 〈

关 键 词：自体骨 种植 骨缺损 移植 组织构建 生物材料 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]