内镜细胞刷几种低温灭菌方法的实验研究  

An Experimental Research of Low Temperature Sterilization Methods for Endoscopic Brushing Cells

作　　者：黄瑞娟[1] 魏明[1] 李月玲[1] 李线明[1] 赵红斌[1] 李党育[1] 

机构地区：[1]广西壮族自治区南溪山医院感染管理科,广西桂林541002

出　　处：《医疗装备》2008年第5期25-26,共2页Medical Equipment

基　　金：广西壮族自治区卫生厅计划课题资助(№Z200613)

摘　　要：目的:探讨几种低温灭菌方法对内镜细胞刷的灭菌效果。方法:内镜细胞刷浸染枯草杆菌黑色变种芽孢(ATCC9372),干燥后分别经戊二醛浸泡3 h、4 h、5 h,戊二醛熏蒸3 h、5 h和环氧乙烷灭菌处理,采样培养7 d。结果:内镜细胞刷经戊二醛浸泡3 h、4 h不能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢,浸泡5 h,则可完全杀灭芽孢,达到灭菌合格;经戊二醛熏蒸3 h、5 h,不能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢;经环氧乙烷灭菌,能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢,达到灭菌合格。结论:戊二醛浸泡5 h灭菌和环氧乙烷气体灭菌可有效杀灭内镜细胞刷污染的细菌芽孢,达到灭菌效果。Objective： To explore the efficient of low Temperature sterile methods for endoscopic brushing cells. Methods： The contaminated brushing cells with Bacillus vat. niger spore were sterilised by six methods： A dipping in glutaraldehyde for 3h, B dipping in glutaraldehyde for 4h, C dipping in glutaraldehyde for 5h, D fumigated with glutaraldehyde for 3h, E fumigated with glutaraldehyde for 5h, F sterilized with ethylene oxide.The brushing cells were sampled and did gennieulture for 7d.Results： The sterilization methods of A, B, D and E cannot killed Bacillus vat.niger spore contaminated on brushing cells, but the meteds of C and D can do.Condusion： Two sterihzation methods that dipping in dipping in glutaraldehyde for 5h and sterilized with ethylene oxide can kill all the spores of bacillus vat. niger contaminated on endoscopic brushing cells.

关 键 词：内镜细胞刷 灭菌 戊二醛 环氧乙烷 熏蒸灭菌 

分 类 号：TH771.4[机械工程—仪器科学与技术]